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从猪废水中培养的微藻中轻度的一步蛋白质恢复,使用天然的基于溶剂的水性双相系统
David Moldes1,2, Marisol Vega1,2, Silvia Bolado1,3
1Institute of Sustainable Processes, University of Valladolid, 47011 Valladolid, Spain.
Molecules (Basel, Switzerland)
|February 13, 2026
概括
在水性双相系统 (ATPS) 中使用的天然深层水溶剂 (NADES) 提供了一种绿色的方法,可以从废水中生长的微藻中提取蛋白质. 贝他因:levulinic酸NADES在短短30分钟内实现了高效的蛋白质恢复和高选择性.
科学领域:
- 生物技术是生物技术.
- 绿色化学 绿色化学
- 废水处理 废水处理
背景情况:
- 在光生物反应器中培养微藻可以处理废水,并产生有价值的生物质.
- 蛋白质是微藻的关键组成部分,使其成为生物技术应用的目标.
- 开发高效和可持续的蛋白质提取方法至关重要.
研究的目的:
- 评价水性双相系统 (ATPS) 中的天然深层环氧溶剂 (NADES),用于从微藻中单步提取蛋白质.
- 为了比较不同基于NADES的ATPS的效率和选择性.
- 为了确定绿色蛋白质回收的最佳条件.
主要方法:
- 使用两个NADES (贝他因:levulinic 酸和胆:urea) 及其组分与酸盐制备了六种ATPS.
- 蛋白质回收率 (PRY) 和选择性 (R) 在不同提取时间的室温下进行评估.
- 基于NADES的ATPS因其在从猪废水中的微藻中恢复蛋白质的潜力而具有特征.
主要成果:
- 在30分钟内,ATPS {betaine:levulinic acid + K3PO4 + H2O} 显示了16.4%的PRY和高选择性 (R = 2.17 g·g-1).
- 蛋白质主要在富含NADES的阶段被回收,在富含盐的阶段存在的最小.
- 虽然单独贝他因产生了最高的PRY (12.2%在120分钟),但贝他因:酸ATPS提供了更好的效率和时间平衡.
结论:
- 基于NADES的ATPS显示出作为有效从微藻中恢复蛋白质的可持续平台的巨大潜力.
- 贝他因:levulinic酸ATPS为蛋白质提取提供了一个有效和更绿色的替代方案,特别是在更短的过程时间.
- 这些系统可用于从营养丰富的废水中培养的微藻中回收蛋白质,为循环经济原则做出贡献.
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