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印ocyanine绿色作为MCF-7乳腺癌细胞中的治疗剂

Wiktoria Mytych¹, Dorota Bartusik-Aebisher², Piotr Oleś³

¹English Division Science Club, Collegium Medicum, Faculty of Medicine, University of Rzeszów, 35-310 Rzeszów, Poland.

Molecules (Basel, Switzerland)

|February 13, 2026

概括

印ocyanine绿色 (ICG) 光动力疗法有效地杀死乳腺癌细胞,同时保存正常细胞. 这项研究证实ICG产生单一氧气,支持其作为乳腺癌治疗的治疗剂的潜力.

科学领域:

生物医学工程生物医学工程
光动力学疗法光动力学疗法
癌症研究癌症研究

背景情况:

印ocyanine绿 (ICG) 是FDA批准的染料,用于瘤成像.
它作为光动力学疗法 (PDT) 的光敏剂的潜力需要进一步研究.
了解ICG在模拟瘤微环境中的有效性至关重要.

研究的目的:

评估ICG对MCF-7乳腺癌细胞的光动力学有效性和选择性.
评估ICG在模拟瘤细胞外基质 (ECM) 扩散的2D和3D细胞培养模型中的性能.
确认单片氧 (1O2) 作为主要细胞毒性机制的直接生成.

主要方法:

在2D和3D原基质中培养了MCF-7乳腺癌细胞和HMEC正常细胞.
细胞被ICG处理,并用780nm二极管激光照射.
细胞活力,ICG吸收,透和1O2生成使用测试,流细胞计,显微镜和光谱计进行了量化.

主要成果:

在二维 (41.7%死亡) 和三维 (29.8%死亡) 培养中,ICG-PDT显著降低了MCF-7细胞活力.
正常的HMEC活力仍然很高 (9%的减少),产生了约4.6.6的治疗指数.
直接光谱证据证实有效的1O2生成,在3D模型中有效性降低,表明透障碍.

结论:

ICG显示显著的光动力学细胞毒性对MCF-7乳腺癌细胞,对2D培养中的正常细胞的影响最小.
1O2生成的直接光谱确认支持其在ICG-PDT细胞毒性作用中的作用.
该研究强调了ECM透障碍在3D瘤模型中的重要性,并支持ICG作为乳腺癌治疗药物的潜力.

关键词:

在二维细胞培养中三维细胞培养的3D细胞培养在MCF-7中使用.乳腺癌乳腺癌乳腺癌印度氨酸绿色的绿色接近红外线的近红外线光动力学疗法是一种光动力学疗法.一个单一的氧气.

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