冷却不会影响活猪精子中细胞内储存的功能,在液体保存过程中
Doanh H Bui1,2, Anne-Marie Luther1, Dagmar Waberski1
1Unit for Reproductive Medicine, Clinic for Swine and Small Ruminants, University of Veterinary Medicine, 30559 Hannover, Germany.
International journal of molecular sciences
|February 13, 2026
概括
将野猪精子冷却到5°C以进行储存会影响初始水平,但不会影响重新加热后细胞内储量的释放. 这一发现对于辅助生殖技术至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 细胞内 (Ca2+) 调节对于哺乳动物的精子功能和生育能力至关重要.
- 精子处理,包括冷却,可以破坏平衡,可能影响辅助生殖的结果.
研究的目的:
- 研究将野猪精子冷却到5°C并随后储存对细胞内Ca2+储存功能的影响.
- 评估储存温度 (5°C与17°C) 和时间对野猪精子Ca2+动态的影响.
主要方法:
- 野猪精液被存储在BTS扩展器中,在5°C或17°C下保存长达五天.
- 细胞内Ca2+水平使用Fluo-4/AM和流细胞计测量.
- 在重新加热后,监测了Ca2+储存对硫醇刺激的反应.
主要成果:
- 存储在17°C的精子显示出较高的初始自由细胞内Ca2+水平,而不是存储在5°C的精子 (p<0.05).
- 重温至38°C消除了储存组之间的Ca2+水平差异.
- 蒂默索尔诱导的Ca2+释放动力学不受储存温度或持续时间的显著影响,除了5°C样本5日的短暂增加外.
结论:
- 猪精子的冷却和储存不会影响重新加热后细胞内Ca2+储存的释放动态.
- 这些发现表明,目前涉及冷却的精液加工方法与维持精子细胞内Ca2+存储功能相兼容.
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