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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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Elements and Compounds

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Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
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Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
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Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
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Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
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在病毒感染期间,单细胞RNA-Seq对人类外周血液细胞中可移植元素表达的剖析.

Oleg D Fateev1, Vasily E Akimov1, Olga V Glushkova1

  • 1Federal State Budgetary Institution "Center for Strategic Planning and Management of Biomedical Health Risks" of the Federal Medical-Biological Agency (FSBI "CSP" of FMBA of Russia), Pogodinskaya str. 10, bld. 1, Moscow 119435, Russia.

International journal of molecular sciences
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PubMed
概括

可转移元素 (TEs) 调节免疫力. 这项研究在HIV和SARS-CoV-2等病毒感染中发现了独特的非LTR TE表达模式,有助于疾病评估和识别潜在的生物标志物.

关键词:
在 COVID-19 疫情中,艾滋病病毒 艾滋病病毒 艾滋病病毒甲型流感是一类流感.这就是scRNA-seqq.可转移的元素可以转移.病毒感染 病毒感染

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科学领域:

  • 免疫学 免疫学 免疫学
  • 基因组学就是基因组学.
  • 病毒学 病毒学

背景情况:

  • 可移植元素 (TE) 对于免疫调节至关重要,在病毒感染期间活性增加.
  • 非LTR TEs (LINE,SINE,SVA) 显示了与抗病毒反应相关的改变表达.

研究的目的:

  • 在A型流感,HIV和SARS-CoV-2感染期间,研究人类PBMC中的非LTR TE转录密码.
  • 确定细胞特异性TE模式,用于疾病严重程度评估,进展预测和治疗评估.
  • 分析细胞毒性T细胞中的TE联合表达网络,以找到诊断和治疗点.

主要方法:

  • 在98名患者样本上进行单细胞RNA测序 (scRNA-seq).
  • 转录基因组数据的生物信息分析.
  • 对TEs和转录因子的共同表达网络分析.

主要成果:

  • 在HIV和SARS-CoV-2队列中发现了独特的细胞特异性非LTR TE表达模式.
  • 与疾病严重程度,进展和治疗疗效相关的TE表达.
  • 前体细胞毒性T细胞表现出最可变的TE表达,这取决于病毒和疾病的严重程度.
  • 在TE网络中的关键监管参与者被确定为潜在的生物标志物.

结论:

  • 非LTRTE参与调解抗病毒反应.
  • 细胞特异性TE特征可以区分疾病的严重程度并预测疾病的进展.
  • 已识别的TE相关网络和转录因子为病毒感染提供了潜在的诊断生物标志物和治疗点.