在不同的注射策略下,在葡萄木发酵期间基于流细胞计的微生物动力学的监测
Federico Sizzano1, Valentina Bianconi1, Eddy Dorsaz2
1Oenology Research Group, Division of Plant Production Systems, Agroscope, 1260 Nyon, Switzerland.
International journal of molecular sciences
|February 13, 2026
概括
流细胞计 (FCM) 提供了实用的,近乎实时的微生物动态在葡萄酒厂规模的葡萄汁发酵的监测. 这种方法有助于评估酵母和细菌,支持酒策略和风险管理.
科学领域:
- 葡萄酒学 葡萄酒学 葡萄酒学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 酒厂规模的发酵监测对于葡萄酒的质量至关重要.
- 微生物分析的传统方法可能耗时.
- 了解微生物动力学影响葡萄酒制作协议.
研究的目的:
- 为了评估流细胞计 (FCM) 在葡萄末发酵期间监测微生物种群.
- 为了比较FCM在不同酒协议中的有效性.
- 评估FCM在酒中的近实时洞察力的实用性.
主要方法:
- 流细胞计 (FCM) 的应用,以监测酵母活力和细菌种群.
- 分析Pinot Noir葡萄末在三个协议下发酵的分析:酵母注射,Pied-de-Cuve和自发.
- 基于Amplicon的DNA测序用于微生物社区的表征.
主要成果:
- FCM有效评估了酵母的生存能力和代谢活性.
- 在酒精发酵过程中检测和监测了活跃的细菌种群.
- FCM模式与安普利康序列数据部分相关,反映了微生物社区的变化.
- 观察到对微生物动态的协议特异性影响.
结论:
- 流细胞计 (FCM) 是一种实用的工具,用于实时监测酒中的微生物动态.
- FCM为优化发酵监测和风险管理提供了有价值的数据.
- 结合FCM与测序提供了全面的微生物洞察力.
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