亚致杀伤性抗生素暴露诱导了致病性Vibrio parahaemolyticus中诺抗性的微演变
Qian Wu1,2, Han Yang1,2, Tianming Xu1,2
1College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
International journal of molecular sciences
|February 13, 2026
概括
水产养殖中的微致命抗生素残留物迅速诱导Vibrio parahaemolyticus中的诺耐药性. gyrA,parC和gyrB基因中的关键突变驱动了这种获得的抗性,影响了海鲜安全.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 遗传学 遗传学 是一个
背景情况:
- 水生环境是抗生素耐药性发展的水库.
- 维布里奥帕拉海莫利蒂库斯 (Vibrio parahaemolyticus) 是一种海鲜传播的重要病原体.
- 昆类抗生素被广泛使用,导致环境残留.
研究的目的:
- 为了研究Vibrio parahaemolyticus中诺抗性的微演变途径.
- 为了确定潜在的获得抗生素耐药性的分子机制.
- 评估亚致命抗生素压力对细菌适应的影响.
主要方法:
- 在体外诱导耐药性使用亚致死的levofloxacin度.
- 对抗生素敏感性和生物膜形成的表型分析.
- 全基因组测序用于识别遗传突变.
主要成果:
- 列沃素诱导产生了抗药突变 (VPD14M),具有对安培素和西普罗素的交叉耐药性.
- 突变者表现出较低的生长率和增强的生物膜形成.
- 在昆抗性确定区域 (QRDRs) 内的 gyrA (Ser83Ile),parC (Ser85Phe) 和 gyrB (Pro748Ser) 中发现了关键点突变.
结论:
- 杀性类抗生素水平迅速驱动Vibrio parahaemolyticus中的耐药性.
- 特定的QRDR突变是获得醇耐药性的关键驱动因素.
- 这些发现支持加强对海鲜和水产养殖环境中抗生素耐药性的监测.
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