揭示了一种与白内障相关的素酸-MAPK/AP-1纤维化轴在人类状网状细胞中,并通过Diospyros kaki的叶子提取物对其进行调节
Youngsic Jeon1, Hyukjoon Kwon1, Hong Ryul Ahn1
1Center for Natural Product Efficacy Optimization, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea.
International journal of molecular sciences
|February 13, 2026
概括
迪奥斯皮洛斯卡基叶 (EEDK) 的乙醇提取物可以减少眼睛状网 (TM) 中的纤维化. 这种提取物抑制了与眼有关的途径,提供了潜在的新治疗策略.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 玻璃眼症与尾管网 (TM) 中的纤维化重塑有关.
- Lysophosphatidic 酸 (LPA) 在人类TM (HTM) 细胞中强烈促进纤维化和上皮-介质细胞过渡 (EMT).
- 对天然化合物进行抗纤维素性质的研究对于绿眼治疗至关重要.
研究的目的:
- 为了确定是否Diospyros kaki叶 (EEDK) 的乙醇提取物可以减少HTM细胞中LPA诱导的纤维化.
- 探索EEDK发挥其抗纤维素作用的潜在分子机制.
- 为了确定EEDK抗纤维活性的最佳乙醇度.
主要方法:
- 用LPA刺激HTM细胞,并用不同度的EEDK.EThanol处理.
- 分析了ECM组件和EMT标记物的基因和蛋白质表达.
- 细胞迁移被评估使用伤口愈合试验.
- 研究了MAPK/AP-1信号通路和转录因子活性.
主要成果:
- 70%的乙醇EEDK提取物显示出显著的抗纤维活性,减少ECM沉积和EMT标记.
- 这种提取物剂量依赖地抑制了LPA诱导的HTM细胞迁移.
- 由LPA诱导的MAPK/AP-1信号的激活被70%的乙醇EEDK抑制,类似于MAPK路径抑制剂.
- 90%的乙醇提取物显示出最小的抗纤维菌作用.
结论:
- 70%乙醇EEDK有效地减轻LPA诱导的TM纤维化和EMT.
- 抗纤维化机制涉及抑制MAPK/AP-1-介导的转录.
- 埃德克显示出作为治疗青光眼治疗的治疗剂的潜力.
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