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介质干细胞衍生的外体在宫癌球状体中重编程化学敏感性途径

Piyatida Molika¹, Kesara Nittayaboon¹, Kankamol Kerdkumthong¹

¹Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand.

International journal of molecular sciences

|February 13, 2026

概括

介质细胞干细胞衍生外体 (MSC外体) 对宫癌 (CC) 化疗敏感性表现出双重影响. 使用MSC外体的预处理可以根据细胞类型和使用的药物来提高或降低治疗疗效.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

宫癌 (CC) 构成了全球严重的健康负担,常常因化疗耐药性和复发而复杂化.
介酶干细胞衍生的外体细胞 (MSC外体细胞) 已经表现出双重能力,可能作为治疗剂,同时也有助于化学抵抗.
在宫癌中调节化疗反应的MSC外体的确切作用仍然不完全理解.

研究的目的:

研究MSC外体细胞预处理对宫癌细胞对化疗敏感性的影响.
在三维 (3D) 子宫癌球形模型中探索MSC外体对化学抵抗和化学敏感性的上下文依赖的影响.

主要方法:

从HeLa和SiHa宫癌细胞系生成3D球形模型.
对MSC外体的蛋白质组分析,以确定关键的相关蛋白质.
评估MSC外基因组预处理对球体敏感性对帕克利塔塞尔与西斯普拉丁或卡博普拉丁联合治疗的影响.

主要成果:

蛋白质组分析确定了MSC外体中的ANXA1,ANXA2,EEF2,LGALS1和PKM2等蛋白质,与瘤再生和化疗反应有关.
在MSC外基因组中,药物排放的上下文依赖调节,代谢重编程,应激适应,亡,DNA损伤反应和整合素信号传递得到了证明.
通过MSC外基因组进行预处理,显著增强了HeLa球体中的化疗诱导的细胞毒性,增加了细胞亡.
SiHa球体表现出选择性反应,MSC外基因组增强了对帕克利塔塞尔-碳波拉丁的敏感性,但不是对帕克利塔塞尔-西斯普拉丁的敏感性,特别是在球体核心.

结论:

在MSC外基因组预处理对子宫癌球体具有细胞类型特异性和药物特异性影响.
这些发现支持MSC外体在宫癌中调节化疗反应的潜力,为新的治疗策略提供了途径.

关键词:

灭症 (apoptosis) 是一种死亡的过程.宫癌:子宫癌是一种癌症.化疗化疗是一种化学疗法.药物耐药性耐药性药物耐药性外基因组是外基因组的组成部分.介质细胞干细胞介质细胞干细胞球形状的球形体是指球形状的球体.

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