生物分析UHPLC-MS/MS方法,用于测量单口分娩研究中的特比纳的量化
Benjamin Rossier1,2, Emmanuel Varesio1,2, Eric Allémann1,2
1School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland.
Biomedical chromatography : BMC
|February 13, 2026
概括
一种新的UHPLC-MS/MS方法量化了猪中的特比纳,一个指甲模型. 这种经过验证的测定方法具有敏感性和特异性,有助于研究用于治疗指甲真菌感染的局部特比纳芬输送.
科学领域:
- 分析化学 分析化学
- 药理动力学 药理动力学
- 皮肤病学 皮肤病学
背景情况:
- 特尔比纳芬是治疗指甲真菌感染 (onychomycosis) 的主要治疗方法.
- 准确量化指甲组织中的特比纳对于开发有效的局部治疗至关重要.
- 猪可以作为人类指甲研究的可行,经济高效的模型.
研究的目的:
- 开发和验证一种快速,选择性和敏感的超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 方法.
- 在猪中量化terbinafine的度.
- 评估该方法对评估局部特比纳芬配方的实用性.
主要方法:
- 优化了UHPLC-MS/MS分析的染色学和检测参数.
- 使用甲醇:水溶剂系统评估了terbinafine提取效率.
- 根据标准操作程序验证分析方法,使用terbinafine-d3作为内部标准.
- 应用该方法来量化用局部配方处理的猪,包括激光切除的样本中的特比纳.
主要成果:
- 使用MeOH:H2O (9:1) 提取溶剂实现了~90%的特尔比纳回收.
- 经过验证的方法证明了对1-200 ng/mL之间的度的敏感性,运行时间为7.2分钟,量化极限为1 ng/mL.
- 在完整的和激光切除的猪中成功检测到特比纳,其沉积量分别为89.04±4.02μg/cm2和217.49±8.98μg/cm2.
结论:
- 开发的UHPLC-MS/MS方法是敏感的,有选择的,快速的.
- 这种方法适合在猪中量化terbinafine.
- 该试验可以支持进一步的研究,以增强当前的terbinafine交付治疗桃菌的治疗.
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