相关实验视频

Updated: Feb 14, 2026

Production and Titering of Recombinant Adeno-associated Viral Vectors
08:35

Production and Titering of Recombinant Adeno-associated Viral Vectors

Published on: November 27, 2011

62.7K

在CHO细胞中通过MAR增强的转位子载体与布拉斯蒂奇丁选择相结合,有效地产生复合mAbs

Xiaoyin Wang1,2, Xuelian Han1, Ting Liu1

  • 1School of Basic Medical Sciences, Henan Medical University, Xinxiang 453003, China.

Acta biochimica et biophysica Sinica
|February 13, 2026
PubMed
概括

一种基于MAR的新型piggyBac转位子系统显著增强了CHO细胞中复合抗体的产生. 这种方法提高了克隆选择效率,从而提高了治疗性抗体制造的产量和长期表达稳定性.

关键词:
这就是CHO细胞.抗体是一种抗体.这是一个神圣的 blasticidin.矩阵连接区域是矩阵连接区域.在 piggyBac 转位子上

更多相关视频

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis08:57

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Published on: March 10, 2014

12.8K
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems13:47

Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Published on: April 23, 2012

11.4K

相关概念视频

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.6K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
19.4K
Production Efficiency01:01

Production Efficiency

Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
18.6K
Vector Product (Cross Product)01:17

Vector Product (Cross Product)

Vector multiplication of two vectors yields a vector product, with the magnitude equal to the product of the individual vectors multiplied by the sine of the angle between both the vectors and the direction perpendicular to both the individual vectors. As there are always two directions perpendicular to a given plane, one on each side, the direction of the vector product is governed by the right-hand thumb rule.
Consider the cross product of two vectors. Imagine rotating the first vector about...
28.1K
Recombinant DNA01:09

Recombinant DNA

Overview
103.5K
Scalar and Vector Triple Products01:06

Scalar and Vector Triple Products

Two vectors can be multiplied using a scalar product or a vector product. The resultant of a scalar product is scalar, while with vector products, the resultant is a vector. These rules of the scalar or vector product between two vectors can be applied to multiple vectors to obtain meaningful combinations. The scalar triple product is the dot product of a vector with the cross product of two vectors.
The scalar triple product is the dot product of a vector with the cross product of two vectors....
4.5K