内生核桃聚醇对核桃粉分离蛋白的结构和功能性质的影响
Yunhua Cao1, Yanmei Deng1, Guohui Yuan1
1College of Biological and Food Engineering, Southwest Forestry University, Kunming 650224, China.
Food chemistry: X
|February 13, 2026
概括
核桃皮质聚醇 (WPP) 显著影响核桃蛋白分离物 (WalPI) 的结构和功能. 删除WPP可以改善水和泡,但会减少油,乳化能力和稳定性.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 蛋白质化学 蛋白质化学
背景情况:
- 核桃蛋白分离物 (WalPI) 是一种有价值的食品成分.
- 内生核桃聚醇 (WPP) 可能会影响WalPI的特性.
- 了解WPP-WalPI相互作用对于食品加工至关重要.
研究的目的:
- 调查WPP对WalPI结构和功能的影响.
- 阐明 WPP-WalPI 相互作用背后的机制.
- 为优化核桃衍生成分在食品应用中提供见解.
主要方法:
- 核桃粉 (WM) 处理以调整多含量.
- 分析蛋白质结构 (β片,随机卷) 和展开.
- 测量功能性质 (持水性,泡性,持油性,乳化能力,溶解性,热稳定性).
- 显微镜 (SEM) 和分子对接模拟.
主要成果:
- 在WalPI中,WPP降低了β-sheet含量,增加了随机卷轴结构,诱导了展开.
- 水容量从1.72g/g增加到2.48g/g;泡容量和稳定性分别提高了31.12%和46.44%.
- 表面疏水性降低了62.9%;油容量,乳化容量,溶解度和热稳定性降低了.
结论:
- WPP显著改变了WalPI结构,影响了其功能性质.
- 由WPP引起的变化增强了一些功能 (水化,发泡),同时减少了其他功能 (乳化,稳定性).
- 结果提供了基于多含量在食品加工中利用WM和WalPI的策略.
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