相关实验视频

Updated: Feb 14, 2026

A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis

A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis

Published on: October 24, 2015

对RNA修饰酶的多omics表征确定了NAT10作为肝细胞癌中功能验证的预后生物标志物

Qianqian Zhan^1,2,3, Huihui Sun^2,3, Xiangting Wang^2,3

¹Division of Life Sciences and Medicine, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.

Frontiers in immunology

|February 13, 2026

概括

这项研究揭示了在癌症中广泛存在的RNA修饰酶 (RME) 失调,与副本数量变化有关. 一个12RME签名和6基因模型提供诊断和预后价值,NAT10显示在肝癌中的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

RNA修饰酶 (RME) 是重要的转录后调节剂.
它们的失调与癌症的发病和进展有关.
全球模式和RME在癌症中的临床相关性尚未完全理解.

研究的目的:

分析RME表达,拷贝数变化 (CNV) 和多种癌症类型的临床结果.
使用机器学习识别瘤歧视RME签名.
研究瘤微环境中的RME作用,并预测药物敏感性.

主要方法:

对RME表达和CNV的综合性多omics分析.
机器学习用于RME签名识别.
单细胞RNA测序 (scRNA-seq) 用于瘤微环境分析.
开发和验证LASSO衍生的预后模型.
药物敏感性预测和功能性测试 (EdU,qRT-PCR,IHC). 药物敏感性预测和功能性测试 (EdU,qRT-PCR,IHC). 药物敏感性预测和功能性测试 (EdU,qRT-PCR,IHC). 药物敏感性预测和功能性测试 (EdU,qRT-PCR,IHC). 药物敏感性预测和功能性测试.

主要成果:

在癌症中,RME被广泛上调,与CNV增长有关.
一个12-RME签名有效地区分瘤和正常组织.
单细胞分析揭示了瘤透细胞中的RME丰富,T细胞亚群中的特定模式.
一个6基因预后模型证明了独立的预后能力.
功能性测试表明NAT10在肝细胞癌 (HCC) 扩散中的潜在作用.

结论:

跨癌症的RME失调的CNV相关景观被描绘出来.
建立了一个12RME诊断签名和一个6基因预后模型.
单细胞分析提供了关于RME表达异质性的见解.
这些发现为了解RME在癌症进展和临床分层中的作用提供了一个框架.

关键词:

在 NAT10 中,NAT10 包含了NAT10.RNA修饰酶是RNA修饰酶的重要组成部分.副本编号变化副本编号变化肝细胞癌是肝细胞癌.免疫微观环境免疫微环境预后签名预测签名单细胞转录组学单细胞转录组学瘤免疫疗法免疫疗法

更多相关视频

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

相关概念视频

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

Enzymes

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

Reliability and Validity

Reliability and Validity

Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.