Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Structure01:23

RNA Structure

79.2K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.2K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

27.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.2K
RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.1K
RNA Stability01:53

RNA Stability

35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
RNA Splicing01:32

RNA Splicing

60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Epigenome-wide association study meta-analysis of wellbeing.

Clinical epigenetics·2026
Same author

In-depth Human Phenotype Ontology Curation Boosts Prioritization Performance for Netherton Syndrome.

The British journal of dermatology·2026
Same author

DNAm landscape up to 4 months post SARS-CoV-2 infection: insights from four population-based cohorts.

Clinical epigenetics·2026
Same author

A multidisciplinary RNA-guided approach to complement genomic analysis of unsolved patients with an inborn error of immunity.

Frontiers in immunology·2026
Same author

Managing non-SCID T cell lymphopenia after TREC-based newborn screening.

Journal of human immunity·2026
Same author

<i>Trans</i>-eQTLs reveal the architecture of human gene regulatory networks.

medRxiv : the preprint server for health sciences·2026

相关实验视频

Updated: Feb 14, 2026

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K

通过通过可解释和交互的RNA引导工作流来链接DNA和RNA来加速罕见疾病诊断.

Willem T K Maassen1,2, Charlotte C E T Pape2,3, Carlos G Urzua-Traslavina2,4

  • 1Genomics Coordination Center, University Medical Center Groningen, Antonius Deusinglaan 1 9713 AV, Groningen, The Netherlands.

NAR genomics and bioinformatics
|February 13, 2026
PubMed
概括

这项研究引入了一个新的RNA引导工作流来管理RNA测序数据的变异,改进了罕见疾病的基因疾病关联分析. 工作流程有助于确定遗传变异,并支持临床解释以更好地诊断.

更多相关视频

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.6K

相关实验视频

Last Updated: Feb 14, 2026

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.7K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.6K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 医学遗传学 医学遗传学

背景情况:

  • 由于生物和技术的变化,RNA测序 (RNA-seq) 在基因组诊断中面临着挑战.
  • 随着时间的推移,从不同来源解释RNA-seq数据是复杂的,阻碍了临床应用.
  • 现有的机器学习方法提供部分校正,但不能完全解决解释复杂性.

研究的目的:

  • 开发一个全面的RNA引导工作流程,以解决RNA-seq数据的变化.
  • 为了在罕见病患者中实现准确的基因疾病关联分析.
  • 为了简化临床决策的变异解释.

主要方法:

  • 开发了一个新的RNA引导工作流程,集成了OUTRIDER,FRASER,Borzoi和MOLGENIS VIP.
  • 实施了一种简化流程,用于处理RNA-seq数据中的生物和技术变异.
  • 用于罕见疾病队列的基因组,表型和分离分析.

主要成果:

  • 该工作流通过管理数据变异,成功识别基因疾病关联.
  • 交互式报告可视化异常基因,并优先考虑临床解释的患者水平变异.
  • 对144例病例的分析表明,增强了变异解释,并有助于临床决策.

结论:

  • 该RNA引导的工作流有效地处理变异,促进基因疾病关联的发现.
  • 它加速了遗传变异的优先级和重新分类,包括未知意义的变异.
  • 这种方法支持临床解释和RNA-seq在诊断中的主流采用.