幼虫作为用于检测高度致病性禽流感A(H5N1) 病毒的替代样本
Tabitha C Viner1, Dawn Dirks2, Dyan J Straughan1
1National Fish and Wildlife Forensic Laboratory, U.S. Fish and Wildlife Service, Ashland, OR, USA.
概括
吹幼虫 () 可用于检测高致病性禽流感 (HPAI) 在刚尸体当传统尸体解剖延迟时. 这种方法在具有挑战性的情况下为诊断HPAI提供了可行的替代方案.
科学领域:
- 兽医病理学 兽医病理学
- 野生动物疾病生态学 野生动物疾病生态学
- 法医昆虫学 法医昆虫学
背景情况:
- 经过改变的动物尸体由于分解和食而带来了重大诊断挑战.
- 在延迟尸体解剖病例中确定感染性死亡原因往往受到组织退化所阻碍.
- 高致病性禽流感 (HPAI) 疫情需要快速准确的诊断方法.
研究的目的:
- 评估吹幼虫 () 作为检测HPAI A(H5N1) 病毒的替代样本的实用性.
- 为了确定HPAI A(H5N1) 是否可以检测到来自加利福尼亚体分解的幼虫.
- 评估使用昆虫幼虫在野生动物死亡事件中的死后诊断的可行性.
主要方法:
- 在HPAI疫情爆发期间,采集了加利福尼亚康多尔大约16天死亡后的尸体样本.
- 在尸体上发现的第三级吹幼虫被收集并冷.
- 使用RT-PCR检测HPAI A(H5N1) 病毒检测在康多尔脏组织和采集的幼虫上进行.
主要成果:
- 在的脏组织中检测到HPAI A(H5N1) 病毒 (Ct值15.7).
- 在收集的吹幼虫 (Ct值32) 中也检测到HPAI A(H5N1) 病毒.
- 这些发现表明,从明显分解的尸体中的幼虫中存在病毒RNA.
结论:
- 吹幼虫可以作为可接受的替代样本用于HPAI A ((H5N1) 检测,当理想的组织不可用或降解时.
- 这种方法为在延迟尸体解剖的野生动物死亡情况中诊断HPAI提供了潜在的解决方案.
- 在解释负结果时建议谨慎,因为HPAI病毒在幼虫中的存在可能是暂时的.
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