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开发一种基于aptamer的快速诊断试验,用于使用全细胞SELEX技术检测沙门氏菌

Abebe M Aga^1,2, Demise Mulugeta¹, Abera Motuma¹

¹Armauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.

Microbiology spectrum

|February 13, 2026

概括

使用银纳米颗粒的新的aptamer-based诊断试验提供了快速和灵敏的沙门氏菌检测. 这种低成本的方法对食品安全和公共卫生至关重要,特别是在资源有限的地区.

科学领域:

生物技术是生物技术.
纳米技术纳米技术
食品安全食品安全

背景情况:

快速而准确的沙门氏菌检测对于食品安全和减少肠道感染至关重要,特别是在资源不足的环境中.
基于aptamer的诊断对基于抗体的方法具有优势,因为其特异性,稳定性和成本.
现有的诊断方法在资源有限的环境中往往无法使用或耗时.

研究的目的:

开发一种快速,低成本和灵敏的基于aptamer的色度测量诊断试验,用于检测沙门氏菌.
通过通过指数式丰富 (SELEX) 选择的DNA吸收体,与银纳米粒子 (AgNPs) 结合.
为了使现场水平的病原体识别能够轻松地进行视觉检测,并支持 Point-of-Care 应用.

主要方法:

针对Salmonella enterica的DNA受体被使用SELEX进行了选择.
银纳米粒子 (AgNPs) 被合成并与候选体结合.
使用暴露于不同沙门氏菌度的阿普坦-AgNP复合体进行了点点色度测试,并通过光谱测量量量了聚合量.

主要成果:

选择阿普塔默显示了渐进的丰富,在12轮SELEX后观察到显著的结合和聚合.
采用aptamer-AgNP测定方法,在10^5 CFU/mL时可视检测出沙门氏菌,从10^5 CFU/mL时进行光谱光度检测.
该试验表明,对沙门氏菌与大肠杆菌有选择性的结合,其聚合反应取决于度.

结论:

这项研究成功地证明了阿普他默结合的AgNP色度测试对敏感和特定的沙门氏菌检测的诊断适用性.
开发的测定方法是沙门氏菌诊断的一个有希望的,负担得起的替代方案,适合低资源环境.
未来的工作应该集中在增强用于侧向流量测定适应和护理点应用的aptamer固定.

关键词:

这是一种DNA吸收剂.沙门氏菌检测沙门氏菌检测颜色测量试验的测试方法纳米颗粒是一种纳米粒子.医疗点诊断的诊断方法全细胞SELEX的使用.

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