高亲和度重组抗体使敏感的MRGPRX2特异ELISA能够检测过敏性鼻炎和慢性自发性疹
Chao Wang1, Na Wang2, Yinghong Jin1
1School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
Analytical and bioanalytical chemistry
|February 13, 2026
概括
使用重组抗体的新ELISA测定准确地检测患者血清中的Mas相关G蛋白结合受体X2 (MRGPRX2). 这种方法在过敏性鼻炎和慢性自发性疹中显示MRGPRX2水平升高,有助于诊断巨细胞疾病.
科学领域:
- 免疫学 免疫学 免疫学
- 过敏和临床免疫学
- 生物技术是生物技术.
背景情况:
- 与Mas相关的G蛋白结合受体X2 (MRGPRX2) 对于巨细胞激活和伪过敏反应至关重要.
- 在周围血液中准确检测MRGPRX2对于诊断与大细胞相关的疾病,如过敏性鼻炎 (AR) 和慢性自发性疹 (CSU),至关重要.
- 由于现有的抗体策略的局限性,目前的MRGPRX2测定缺乏准确性,可重现性和标准化.
研究的目的:
- 开发和验证一种新型,敏感和特定的免疫试验,用于检测循环中的Mas相关G蛋白结合受体X2 (MRGPRX2).
- 评估这种测试在区分患有巨细胞相关疾病的患者和健康个体的有用性.
主要方法:
- 动物通过IEDB分析资源识别的合理选择的MRGPRX2 (残留289-330) 进行免疫接种.
- 用测序定义的重组单克隆抗体被生成并使用表面等离子体共振和西欧斑块进行了表征.
- 一种三明治ELISA被开发并使用AR,CSU和健康对照患者的血清样本进行验证.
主要成果:
- 最佳捕获-检测抗体对显示出高亲和力.
- 开发的ELISA表现出极好的线性 (R2=0.9996),令人满意的恢复 (83.82-115.60%),低不精确度 (CVs <6%),以及低检测极限 (0.981 ng/mL).
- 与健康对照人群相比,AR和CSU患者的血清MRGPRX2水平显著更高.
结论:
- 基于重组抗体的ELISA提供了一种敏感,特异,稳定和可重复的方法来检测循环中的MRGPRX2.
- 这种测定具有临床和转化应用在诊断和管理与巨细胞相关疾病的重大潜力.
- 在AR和CSU患者中MRGPRX2水平升高表明它作为这些疾病的潜在生物标志物的作用.
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