在绝对定量尺度上的酵母过氧体蛋白质组
Hirak Das1, Silke Oeljeklaus1, Renate Maier1
1Biochemistry II, Theodor-Boveri-Institute, Faculty of Chemistry and Pharmacy, University of Würzburg, Am Hubland, 97094, Würzburg, Germany.
Histochemistry and cell biology
|February 13, 2026
概括
酵母过氧体在油性生长过程中显著增加了蛋白质丰富度,特别是对于β-氧化酶,展示了有机体的适应. 这项研究量化了不同代谢条件下的过氧体蛋白质组变化.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 过氧体对脂质代谢和氧化还原平衡至关重要.
- 酵母过氧体中的蛋白质表达受到代谢调节.
- 了解过氧体蛋白质组动力学是细胞功能的关键.
研究的目的:
- 在油酸和葡萄糖条件下量化酵母过氧体蛋白质组.
- 为了研究过氧体蛋白丰度的适应性变化.
- 为过氧体生物学研究提供量化资源.
主要方法:
- 无标签的质谱仪用于绝对蛋白质量定量.
- 在不同的代谢状态下 (油酸与葡萄糖) 对Saccharomyces cerevisiae蛋白质组的分析.
- 确定过氧体和相关蛋白质的蛋白质拷贝数.
主要成果:
- 总体而言,油酸盐 (2.8%) 和葡萄糖 (0.8%) 的过氧体蛋白质约为3倍.
- 核心过氧体蛋白显示油酸的9倍增加,支持脂肪酸β-氧化.
- 代谢酶如β-氧化和氧酸盐循环酶增加了500倍以上;进口机械显示适度变化 (2-8倍).
结论:
- 在对代谢线索的反应中,酵母甲氧化体经历了显著的重塑.
- 器官细胞表现出了显著的适应性灵活性,特别是在代谢酶含量方面.
- 这种定量蛋白质组数据集是研究过氧体功能和适应的宝贵资源.
相关概念视频
Peroxisomes and Mitochondria
98.0K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
98.0K
Peroxisomes
21.4K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
21.4K
Yeast Signaling
17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Absolute Value Inequalities
350
The absolute value is a mathematical tool that represents the distance of a number from zero on the number line, regardless of its sign. In the context of inequalities, absolute value expressions help define a range of permissible values or boundaries for a variable. These inequalities are commonly used in scientific modeling and data interpretation, where variability within or beyond a certain threshold must be captured precisely.An absolute value inequality of the form ∣x∣ ≤...
350
Mean Absolute Deviation
3.5K
The mean absolute deviation is also a measure of the variability of data in a sample. It is the absolute value of the average difference between the data values and the mean.
Let us consider a dataset containing the number of unsold cupcakes in five shops: 10, 15, 8, 7, and 10. Initially, calculate the sample mean. Then calculate the deviation, or the difference, between each data value and the mean. Next, the absolute values of these deviations are added and divided by the sample size to...
Let us consider a dataset containing the number of unsold cupcakes in five shops: 10, 15, 8, 7, and 10. Initially, calculate the sample mean. Then calculate the deviation, or the difference, between each data value and the mean. Next, the absolute values of these deviations are added and divided by the sample size to...
3.5K
Protein Import into the Peroxisomes
5.5K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
5.5K


