硫化通过TaATG6c硫化促进小麦对条纹生的免疫力
Ying Ma1, Fangfang Peng1, Ran Zhao2
1College of Life Sciences and State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Stress biology
|February 13, 2026
概括
硫化 (H2S) 通过增强自增强小麦对条纹生的抵抗力. H2S针对TaATG6c蛋白质,促进其功能并改善植物对Puccinia striiformis f. sp. 的免疫力. 三病原体. 的病原体.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 植物生理学 植物生理学
- 生物化学 生物化学
背景情况:
- 硫化 (H2S) 是植物应激反应中的关键气传递物.
- H2S在小麦抵抗条纹 (由Puccinia striiformis f. sp. 引起的) 的特定作用. 三,Pst) 并没有得到很好的理解.
- 自是一种重要的细胞过程,与植物免疫有关.
研究的目的:
- 调查外源H2S在增强小麦对条纹生的抗性方面的作用.
- 为了确定涉及小麦-Pst相互作用的H2S目标.
- 阐明H2S赋予电阻的分子机制.
主要方法:
- 进行比较的持续硫化蛋白质组学,以确定H2S目标.
- 特定地点的质谱和修改的生物素开关试验,以检测蛋白质化.
- 病毒诱导的基因沉默 (VIGS) 和短暂的过度表达,以研究基因功能.
- AlphaFold结构建模用于预测蛋白质-蛋白质相互作用.
- 对自细胞激活标记物的分析 (例如,ATG8脂化).
主要成果:
- 外源H2S显著增强小麦对Pst.的抵抗力.
- 与自相关的蛋白质TaATG6c被确定为直接的H2S标,Cys177和Cys180是关键的化位.
- TaATG6积极调节小麦对Pst的免疫力;它的沉默会损害抵抗力,而过度表达会增强它.
- H2S诱导的TaATG6c的化促进了自的启动,由增加的ATG8脂化证明.
- 在TaATG6c中关键的氨酸残留物的突变减弱了其抵抗传递功能和H2S响应.
结论:
- 硫化通过向和修改TaATG6c.提高了小麦条纹的抗性.
- H2S通过TaATG6c强硫化促进了自的启动,这是植物应激适应的关键氧化还原调节机制.
- 这项研究揭示了一种新的分子途径,该途径将气传递器信号,蛋白质修饰和植物免疫中的自联系起来.
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