验证和临床研究的单步玻璃化结合单步加热的人类胚胎囊
Jan Gunst1, Hubert Joris2, Matthijs Vynck3
1Department of Laboratory Medicine-Centre for Reproductive Medicine, General Hospital Sint-Jan Brugge, Bruges, BE, Belgium. jan.gunst@azsintjan.be.
Journal of assisted reproduction and genetics
|February 13, 2026
概括
单阶段玻璃化和加热 (SSV/SSW) 为人体胚胎囊冷保存提供了一个可行的替代方案. 这种方法减少了处理时间和冷保护剂的暴露,同时与传统的逐步方法相比,保持了类似的持续怀孕率.
科学领域:
- 人类胚胎学 人类胚胎学
- 生殖医学是一种生殖医学.
- 低温生物学 低温生物学
背景情况:
- 人类胚胎囊的冷保存对于辅助生殖技术至关重要.
- 逐步玻璃化和加热 (SWV) 是当前的标准,但涉及多个步骤和更长的暴露时间.
- 之前的动物研究表明,可以使用更短的冷保存方法.
研究的目的:
- 评估单步玻璃化和加热 (SSV/SSW) 对人类胚胎细胞的可行性.
- 在胚胎和临床结果方面,将SSV/SSW与SWV进行比较.
- 确定SSV/SSW是否可以成为SWV的有效替代品.
主要方法:
- 使用研究胚芽细胞的SSV/SSW验证,评估生存,再扩张和发育.
- 在SSV和SWV之后测量冷保护剂 (CPA) 水平.
- 从333个SWV/SSW和568个SSV/SSW胚胎细胞移植中获得的实验室和临床结果的临床应用和比较.
主要成果:
- 在验证过程中,SSV/SSW显示了与SWV相比的崩和重新扩张率.
- 细胞外CPA分析显示,与SWV相比,SSV的细胞内吸收显著较低 (79.3 mg/L与127.3 mg/L).
- 临床数据表明,SSV/SSW的崩和重新扩张率较低,但持续怀孕率相似 (25.9%与26.1%).
结论:
- 单阶段玻璃化和加热 (SSV/SSW) 是一个可行且运行效率高的替代阶段玻璃化和加热 (SWV).
- SSV/SSW 减少了处理时间,CPA 暴露和潜在的操作员错误.
- 该方法保持了可比的持续怀孕率,支持其临床适用性.
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