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Updated: Feb 15, 2026

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关于人类脏管类体的lentiviral工程和多原子表征的协议
Giulia Perticari1, Maroussia M P Ganpat1, Jarno Drost2
1Princess Máxima Center for Pediatric Oncology, Oncode Institute, Heidelberglaan 25, Utrecht 3584 CS, the Netherlands.
STAR protocols
|February 13, 2026
概括
本协议详细说明使用健康的脏器官或管状物来模拟脏疾病. 研究人员可以在这些先进的临床前模型中研究瘤进展和遗传突变.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 癌症生物学 癌症生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 来自正常人体组织的器官是有价值的临床前模型.
- 研究瘤进展需要有效的疾病模型.
- 器官体 (管状体) 为病研究提供了一个有前途的途径.
研究的目的:
- 为使用器官体 (管体) 提供疾病建模的详细方案.
- 建立和维护用于实验的管状体.
- 为了实现下游分析,包括遗传和转录基因分析.
主要方法:
- 从健康组织中建立和通过脏管状体.
- 为了引入遗传修饰,用lentiviral转导进行.
- 用于组织学分析,CUT&RUN和单细胞RNA测序的管状体的准备.
主要成果:
- 该协议提供了一个全面的工作流程,用于管状物生成和操纵.
- 这些方法允许在模型疾病中引入特定突变.
- 管状物被准备用于先进的分子和细胞分析.
结论:
- 该协议促进了器官的使用,用于强大的临床前疾病建模.
- 管体作为一个强大的工具,用于调查脏瘤的进展和遗传改变.
- 描述的技术支持工程器官的全面分子分析.
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