选择一种高 afinity 的 Aptamer 的 Phalloidin 和应用在一个双模式的 LRET/色度测量 Aptasensor
Ali Raza1,2,3, Faizan Ul Haq1,2,3, Imran Mahmood Khan4
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, P.R.china.
Analytical chemistry
|February 13, 2026
概括
这项研究开发了第一个用于检测毒性化合物法洛伊丁的DNA吸收体 (Apt-PHD). 双模式口味传感器提供快速,灵敏的检测,提高食品安全.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 来自阿曼尼塔的法洛因因因潜在的错误识别而构成重大食品安全风险.
- 现有的法洛伊丁检测方法是有限的,以前没有报道过基于aptamer的系统.
研究的目的:
- 为了分离一个高亲和度的DNA吸收体,用于法洛因的检测.
- 开发一种多功能双模式口味传感器,用于灵敏而可靠的法洛伊丁量化.
主要方法:
- 使用 15 轮捕获-SELEX.EX 选择一个 DNA 合体 (Apt-PHD).
- 使用ITC,SYBR绿色I试验,CD光谱学和分子对接来表征阿巴 - 标相互作用.
- 开发一种双模式的兰化共振能量传输 (LRET) /色度测量感应传感器,使用上转化纳米粒子和金纳米粒子.
主要成果:
- 隔离的Apt-PHD具有高亲和力 (分离常数在nM范围内) 和特异性.
- 双模式的食感传感器实现了较低的检测极限 (12.6 pg/mL用于LRET,18.0 pg/mL用于色度测量).
- 传感器显示出出色的可复制性,对真菌毒素的选择性,以及在样本中精确的恢复.
结论:
- 这项工作介绍了第一个基于aptamer的phalloidin识别系统.
- 开发的双模式光学传感平台能够快速,可靠,并可在现场部署检测小分子毒素.
- 这项技术通过提供敏感的毒素检测工具来增强食品安全监测.
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