对于Bordetella atropi在体内入侵宿主细胞时,需要一种III型分泌系统
Tuan D Tran1, Serena J Meadows-Graves1, Amanda R Haio1
1Department of Biology, San Diego State University, San Diego, California, United States of America.
线虫肠道细胞的侵袭需要一种III型分泌系统 (T3SS). 这一系统与DeiA蛋白和BvgS调节器一起,对于细菌的进入和病变发生至关重要.
科学领域:
- 微生物学 微生物学
- 病原体与宿主相互作用
- 细菌病原发生的细菌.
背景情况:
- Bordetella atropi 是一种细胞内细菌,可以感染 Oscheius tipulae 线虫.
- 之前的工作确定了线条作为宿主细胞内的传播机制.
- 宿主细胞入侵的机制和促成因素以前是未知的.
研究的目的:
- 研究III型 (T3SS) 和VI型分泌系统 (T6SS) 在B. atropi的病原性中的作用.
- 确定导致B. atropi宿主细胞入侵的特定因素.
主要方法:
- 对T3SS和T6SS突变体在入侵和细胞内传播中的比较分析.
- 电子显微镜和共聚焦光显微镜用于观察宿主细胞相互作用.
- 双重转录组学用于在生物体内识别上调基因.
- 对假定效应因子和毒性调节者的基因淘汰研究.
主要成果:
- 对于入侵或细胞内传播来说,T6SSs不是必不可少的.
- 对于肠道细胞入侵,调解宿主细胞突起,需要T3SS.
- 双重转录学揭示了T3SS上调,并确定了潜在的效应因子和BvgS调节器.
- 删除deia减少了入侵事件;删除bvgS取消了入侵事件.
结论:
- 对于B. atropi侵入Oscheius tipulae肠道细胞来说,T3SS非常重要.
- DeiA和BvgS调节器在B. atropi入侵中发挥着重要作用,BvgS可能会调节T3SS活动.
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