在一些内源性真菌中L-asparaginase活性:Glutaminase-free和低尿酶共活性
Zahra Zaeimian1, Khalil-Berdi Fotouhifar1, Mohsen Farzaneh2
1Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
PloS one
|February 13, 2026
概括
染性真菌被选为L-asparaginase的生产,与Alternaria brassicae C显示高活性和最小的协同活性. 推使用矿物盐和酸来生产L-asparaginase.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 酶学 是一种酶学.
背景情况:
- L-阿斯巴拉金酶是癌症治疗中至关重要的酶.
- 内生菌是新型生物活性化合物的潜在来源.
- 研究L-Glutaminase和尿酶等协同活性对于酶的纯度和有效性很重要.
研究的目的:
- 为了评估内性真菌中L-阿斯巴拉金酶的产生.
- 为了评估与L-asparaginase生产一起的L-glutaminase和尿酶的共同活性.
- 确定L-阿斯巴拉金和培养基对酶生产的影响.
主要方法:
- 选62种内性真菌分离物以检测L-asparaginase活性.
- 使用Nesslerization方法量化L-阿斯巴拉金酶活性.
- 通过L-阿斯巴拉金对酶诱导性和基质调节的评估.
- 测试十种不同的培养基对它们对酶生产的影响.
主要成果:
- 在62个分离物中,49个分离物显示了L-阿斯巴拉金酶活性,其中Cladosporium perangustum EL1具有最高的区域指数 (6.58).
- 克拉多斯波里亚克拉多斯波里亚克尔5-2产生了最大的L-阿斯巴拉基纳酶活性 (10.78 U mL-1).
- Alternaria brassicae C表现出高的L-阿斯巴拉金酶活性 (7.07 U mL-1) 与可以忽略不计的L-胺酶和低的尿素酶协同活性 (1.97 U mL-1).
- 发现L-asparaginase的产生是可诱导和基质调节的.
- 矿物盐和酸在所有分离物中都支持L-酸酶的产生,与其他测试介质不同.
结论:
- 内生真菌,特别是Alternaria brassicae C,是生产L-酸酶的有希望的来源.
- 矿物盐和酸是L-酸酶生产介质的有效替代品.
- 这项研究是首次报告从特定植物物种中分离出来的内菌中产生L-asparaginase,并调查它们的协同活性.
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