增强的光谱域相位显微镜通过联合算法-硬件校准进行高灵敏度和广泛的定量相位成像
Optics letters
|February 13, 2026
概括
增强的光谱域相位显微镜 (SDPM) 克服了定量相位成像的局限性. 这种新方法为生物和工业应用中更广泛的测量范围提供了高精度,无偏差的相位重建.
科学领域:
- 光学成像技术的使用.
- 计量学 计量学 计量学
- 生物物理学的生物物理.
背景情况:
- 光谱域相位显微镜 (SDPM) 提供高灵敏度的定量相位成像.
- 传统的SDPM与相梯度和噪声作斗争,由于包裹不匹配和偏差,限制了测量范围.
研究的目的:
- 引入增强的SDPM (eSDPM),一个算法硬件联合校准框架.
- 为了实现高精度,在延长的测量范围内实现无偏差的相位重建.
主要方法:
- 算法改进:对光谱相终点项的明确建模,参考相估计和波数定.
- 硬件校准:可控的光谱截减和边界波长校准,以最大限度地减少偏差和错位.
主要成果:
- eSDPM展示了连续的,无偏差的光路差异重建.
- 在各种样品的广泛测量范围中实现了高精度.
结论:
- eSDPM建立了一个强大的和实用的方法,用于广泛的定量相位成像.
- 同校准框架显著提高了SDPM的可靠性和适用性.
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