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绿色超声波辅助提取用于高效的胡卜素分析卡拉曼西 (Citrofortunella microcarpa) 的情况
Worakan Chutakool1, Kitipong Assatarakul2, Kanet Wongravee3
1Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok 10330, Thailand.
Ultrasonics sonochemistry
|February 13, 2026
概括
这项研究开发了一种可持续的超声波辅助提取 (UAE) 方法,使用绿色溶剂有效地从calamansi水果中分离生物活性胡卜素. 优化的方法确定了独特的胡卜素样式,包括蛋白和β-酸酸.
科学领域:
- 农业化学 农业化学
- 食品科学 食品科学 食品科学
- 绿色化学 绿色化学
背景情况:
- 卡拉曼西 (Citrofortunella microcarpa) 含有丰富的生物活性胡卜素,但缺乏它们的个体识别和有效的提取方法.
- 了解calamansi的胡卜素成分对于其在食品和营养品行业的更广泛利用至关重要.
研究的目的:
- 开发和优化使用超声波辅助提取 (UAE) 和绿色溶剂,为calamansi开发可持续和高效的胡卜素提取方法.
- 为了确定卡拉曼西果汁中存在的特定胡卜素特征.
- 评估开发的提取方法对环境的影响.
主要方法:
- 选七个溶剂系统的胡卜素提取效率,有和没有阿联.
- 使用响应表面方法 (RSM) 优化提取参数 (溶剂比,温度,时间).
- 使用液体色谱-质谱法 (LC-MS) 鉴定胡卜素,并使用AGREE指标评估绿色.
主要成果:
- 一种深度欧性溶剂 (1:1薄荷:香) 和食用油 (向日油) 与阿联显示出高提取效率.
- 最佳条件 (1:2 男人:Cam,39°C,32分钟) 预测了高的总胡卜素含量 (1.87±0.06μg/mL).
- LC-MS分析揭示了黄蛋白,β-密桑丁及其化衍生物 (C10:0C18:0) 在卡拉曼西的独特特征.
结论:
- 阿联与绿色溶剂系统相结合,为从calamansi提取胡卜素提供了一种可持续和实用的方法.
- 开发的方法有效地提取和表征独特的胡卜素,为增加这种水果的使用铺平了道路.
- 与传统的提取方法相比,优化的薄荷:香系统显示出更好的绿色.
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