磁珠辅助提取与ID-LC-MS/MS相结合,用于同时量化人血清中可溶于脂肪的维生素A,D,E和K
Qingqing Pan1, Jing Lin2, Chunlan Tang3
1Reference Laboratory, Medical System Biotechnology Co., Ltd., Ningbo, 315104, Zhejiang, China.
Talanta
|February 13, 2026
概括
一种新的自动化磁性固体相提取方法准确量化了人体血清中的五种脂溶性维生素. 这种高通量技术为临床维生素监测和营养状况评估提供了更快,更可靠的替代方案.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 临床化学 临床化学
背景情况:
- 准确量化脂肪溶性维生素 (A,D2,D3,E,K1) 对于评估营养状况和诊断缺陷至关重要.
- 像液体液体提取 (LLE) 这样的传统方法往往是耗时和劳动密集的.
- 在临床环境中需要高通量,自动化的方法来有效地进行维生素分析.
研究的目的:
- 开发和验证一种高通量,自动化磁性固相提取 (MSPE) 方法,用于同时量化人类血清中五种关键的脂溶性维生素.
- 将开发的MSPE方法与传统的LLE技术的性能进行比较.
- 建立一个强大的分析工具,用于对维生素状况的常规临床监测.
主要方法:
- 合成一种新的功能化基于的磁性核心外材料,具有用于维生素提取的特定亲和力连接体.
- 应用自动化MSPE与同位素稀释液体染色学-并联质谱学 (ID-LC-MS/MS) 结合用于血清样本的制备和分析.
- 用经认证的参考材料和方法比较研究验证方法的特异性,线性,灵敏性,回收,精度和准确性.
主要成果:
- 经过验证的ID-LC-MS/MS方法表现出优异的特异性,灵敏性和线性 (R2>0.995),量化极限较低.
- 获得了很高的恢复率 (90.48101.95%) 和令人满意的精度 (CVs <7.14% intra-day, <4.68% inter-day).
- 自动化MSPE过程将样品准备时间缩短到11分钟,与临床LLE方法良好一致,在外部质量评估方案中表现出色.
结论:
- 开发的自动化MSPE-ID-LC-MS/MS方法提供了一个高度准确,精确和高效的方法,用于同时量化人类血清中的五种脂溶性维生素.
- 这种强大的方法适用于高通量临床实验室,能够准确评估营养状况并促进实验室自动化.
- 该方法在常规维生素监测中比传统的提取技术有了显著的进步.
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