使用最小侵入性N-同位素稀释方法量化 brojlers的内源性总损失
M F Habibi1, S Dorado-Montenegro2, R P Kwakkel3
1Animal Nutrition Group, Wageningen University & Research, P.O. Box 338, 6700 AH Wageningen, the Netherlands; Faculty of Animal Science, Universitas Gadjah Mada, Sleman, Yogyakarta 55281, Indonesia.
Animal : an international journal of animal bioscience
|February 13, 2026
概括
一种新的最小侵入性同位素稀释 (ID) 方法准确量化了 brojlers 的总内源损失 (tENL). 这种使用尿15N丰富的方法,为评估各种饮食中真正的消化能力提供了切实可行的替代方案.
科学领域:
- 动物营养和新陈代谢
- 可消化性和平衡研究研究.
- 同位素标记剂在家禽中的应用
背景情况:
- 精确测量总内源性损失 (tENL) 对于确定肉的饮食真实消化能力至关重要.
- 目前的方法,如酶化化素 (EHC) 和EHC-超 (EHC-UF),对于特定于饮食的tENL量化有局限性.
- 15N同位素稀释 (ID) 方法为测量基底和特定饮食的ENL提供了一个潜在的方法.
研究的目的:
- 为了量化肉的总内源损失 (tENL),使用微侵袭的15N同位素稀释 (ID) 方法.
- 通过将其估计值与传统的EHC和EHC-UF方法进行比较来验证ID方法.
- 评估尿15N-丰富的实用性作为前体池15N-丰富的代理在被养各种饮食的肉中.
主要方法:
- 总共有432只母肉被食不同纤维成分的饮食或含EHC的半合成饮食.
- 一种15N同位素溶液 (牛奶蛋白缩物和化) 从26日至31天口服给肉.
- 收集了排泄物,皮质消化和组织样本进行和15N丰富分析;尿15N丰富作为前体池代理.
主要成果:
- 尿15N丰富迅速达到稳定状态,与组织15N丰富有很好的相关性 (r=0.84-0.90).
- 来自ID方法的LEAL tENL估计值明显低于EHC (-75.6%) 和EHC-UF (-70.3%) 方法的估计值.
- 与EHC/EHC-UF相比,ID方法的结果是与EHC/EHC-UF相比,略低一些的真实乳腺消化率估计 (-1至-4%单位).
结论:
- 最少侵入性15N-同位素稀释方法是一种可行的和实用的工具,用于量化肉的内源性总损失.
- 利用尿路15N-丰富有效地代表了前体池,使tENL在多种食纤维和蛋白质来源中的量化成为可能.
- ID方法提供了一种有价值的替代方法,用于比较总内源性损失,并评估家禽的真实乳腺消化能力.
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