相关实验视频
Updated: Feb 15, 2026

09:25
A Protocol to Acquire the Degenerative Tenocyte from Humans
Published on: June 9, 2018
7.7K
细胞中核图像的变化来源于从肌扩展体迁移的细胞
Eli Heber Martins Dos Anjos1, Maria Luiza Silveira Mello1, Benedicto de Campos Vidal1
1Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP 13083-862, Brazil.
Acta histochemica
|February 13, 2026
概括
肌细胞染色质组织随着肌培养的持续时间而变化. 扩展培养表明染色质紧缩增加和特定的表观遗传标记物转移,这表明生物工程应用的改变基因表达潜力.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
背景情况:
- 肌结构和机械负荷影响纤维细胞表型.
- 拓展培养中的肌细胞随着时间的推移聚集和对齐,重塑原体.
- 了解细胞表观遗传变化对于生物工程应用至关重要.
研究的目的:
- 为了研究细胞衍生细胞的染色质超组织的差异.
- 为了比较来自8天和12天老鼠肌扩展培养的细胞.
- 评估不同的细胞组织是否会影响染色体转录活动.
主要方法:
- 图像分析和免疫光被用来评估染色体特征.
- 评估了Feulgen-DNA数量,DNA甲基化标记物和H3K9乙化.
- 测量了光吸收率,碎形尺寸和色素边缘化.
主要成果:
- 没有观察到Feulgen-DNA,DNA甲基化或H3K9乙化中的差异.
- 在光吸收,碎形尺寸和染色体边缘化方面发现了差异.
- 12天的培养物显示H3K27三甲基化增加和5-基甲基细胞素减少,表明染色质凝结.
- 12天培养还显示H3K4me2和H3K4me3的升高,这表明尽管紧缩,基因表达的潜在变化仍然存在.
结论:
- 从较长的培养物中获得的细胞衍生细胞表现出明显的染色质组织和表观遗传特征.
- 这些发现表明了差异性细胞外基质合成的潜力,并对肌生物工程产生了影响.
- 需要进一步的研究来将这些表观遗传变化与生物工程中的功能结果相关联.
相关概念视频
Cell Migration
6.7K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.7K
Cell Migration
18.9K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.9K
Nuclear Stability
23.4K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
To hold positively charged protons together...
23.4K
Cancers Originate from Somatic Mutations in a Single Cell
15.0K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
15.0K
Nuclear Fusion
33.9K
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
33.9K
Non-nuclear Inheritance
23.3K
Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm—such as chloroplasts and mitochondria—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
23.3K

