黄图调节巨细胞M1/M2两极分化,通过抑制HIF-1α信号通路来缓解DSS和鲁巴巴诱导的结肠炎缺陷感冒模式
Yajuan Chen1, Huilin Wei1, Anlin Dai1
1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China.
黄图 (HTD) 通过调节巨分化,有效治疗性结肠炎 (UC). 它抑制HIF-1α通路,将巨细胞从炎症M1转移到抗炎M2状态,以获得治疗益处.
科学领域:
- 传统中国医学 (TCM) 是一种
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 黄图 (HTD) 是一个经典的TCM配方,历史上用于胃肠道疾病.
- 性结肠炎 (UC) 是结肠的慢性炎症状况.
- 性结肠炎 (DCP-UC) 缺乏感冒模式是一种特定的TCM诊断,需要有针对性的治疗.
研究的目的:
- 研究HTD在治疗DCP-UC.治疗中的疗效.
- 阐明涉及巨细胞两极化和HIF-1α信号通路的潜在机制.
主要方法:
- 已建立的DSS和大黄诱导的DCP-UC小鼠模型和LPS刺激的RAW264.7巨细胞炎症模型.
- 评估HTD对结肠损伤,炎症和巨细胞两极分化的影响.
- 进行了体内巨细胞枯竭和体内实验,使用骨髓衍生巨细胞 (BMDMs),涉及HIF-1α操纵.
主要成果:
- HTD显著缓解了结肠炎症状,并减少了结肠损伤和炎症.
- HTD调节了巨细胞极化,减少了M1标记物和增加了M2标记物.
- 在M1巨细胞中,HTD抑制了HIF-1α的表达,减少了促炎因素,通过HIF-1α激活/过度表达,效应被逆转.
结论:
- 通过抑制HIF-1α信号通路,HTD在DCP-UC中发挥治疗作用.
- 这种抑制促进了巨细胞表型从M1到M2的转变,减少了炎症.
- 高血压症显示出作为一种潜在的治疗策略的希望,用于DCP-UC.
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