从对焦显微镜图像分析中期怀孕小鼠 decidua 的动脉重塑的定量评估
Dema Alghabban1, Alexander J Muller2
1Lankenau Institute for Medical Research, Wynnewood, PA, United States; Drexel University College of Medicine, Philadelphia, PA, United States; Taibah University College of Medicine, Madinah, Saudi Arabia.
瘤和胚胎都需要血液供应,反映胎盘发育. 一种新方法通过测量鼠标 decidua 中的光滑肌肉α动蛋白 (SMA) 来量化螺旋动脉重塑,有助于研究受损血管发育.
科学领域:
- 生殖生物学 生殖生物学
- 血管生物学 血管生物学
- 免疫学 免疫学 免疫学
背景情况:
- 瘤生长和胚胎发育有相似之处,特别是在获得宿主血液供应方面.
- 子宫螺旋动脉重塑对于胎盘血流至关重要,涉及免疫细胞和热囊细胞.
- 损伤的螺旋动脉改造可能会影响怀孕的结果,强调需要使用定量评估方法.
研究的目的:
- 开发和验证一个定量测试,以评估螺旋动脉重塑在决定的基础.
- 为了研究免疫细胞在妊娠期间螺旋动脉重塑中的作用.
- 建立一个客观的方法来评估受损的血管改造.
主要方法:
- 混焦显微镜和免疫光染色在怀孕中期的小鼠概念.
- 使用DAPI用于细胞性,CAV1用于内皮细胞 (ECs) 和SMA用于血管光滑肌细胞 (VSMCs).
- 量化血管壁内SMA的相对百分比,以评估VSMC的存在.
主要成果:
- 正常的螺旋动脉改造的特点是,在妊娠日7.5和9.5.5之间,血管介质中SMA的显著减少.
- 保持SMA表明螺旋动脉改造受损.
- 开发的方法提供了客观的定量数据,关于船壁内的SMA水平.
结论:
- 描述的基于共聚焦显微镜的试验提供了一个客观的方法来量化螺旋动脉重塑.
- 这种测试可以用来评估免疫细胞和其他因素对怀孕期间血管重塑的影响.
- 了解螺旋动脉改造对于生殖健康和癌症研究都至关重要,因为它们具有共同的病理生理过程.
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