使用PacBio HiFi和Hi-C技术组装Microtus fortis的染色体级基因组
Du Zhang1,2, Qi Hu3, Tianqiong He4,5
1Department of Medical Genetics, The Second Xiangya Hospital of Central South University, Changsha, 410011, China.
Scientific data
|February 13, 2026
概括
研究人员已经创建了第一个染色体水平的基因组组合,用于 (Microtus fortis). 这种高质量的基因组资源将促进对其独特特征和动物进化的研究.
科学领域:
- 基因组学就是基因组学.
- 进行比较基因组学.
- 哺乳动物遗传学 哺乳动物遗传学
背景情况:
- (Microtus fortis) 是一个有价值的动物模型,具有独特的生物特征.
- 它对 Schistosoma japonicum 的自然耐药性尤为有趣.
- 遗传研究需要高质量的参考基因组.
研究的目的:
- 为了产生第一个染色体层次的基因组组合Microtus fortis.
- 促进对物种独特表型的遗传研究.
- 为比较基因组学提供一个基础资源.
主要方法:
- 为了基因组组装,PacBio HiFi长读测序被采用.
- 使用Hi-C数据进行染色体水平的支架.
- 进行了BUSCO分析,以评估基因组的完整性.
主要成果:
- 一个2.29Gb的基因组组件,具有出色的连续性 (连接N50=68.89Mb;支架N50=91.23Mb).
- 97.7%的序列固定在26个伪分子上,与型相匹配.
- 鉴定了23678个编码蛋白质的基因,其中97.5%的基因是功能分配的.
结论:
- 高质量的基因组组装是Microtus fortis研究的重要资源.
- 它将使得在天生的免疫力和抗病能力背后的遗传机制的探索成为可能.
- 这次会议将通过比较基因组学增强对动物进化的理解.
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