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相关概念视频

Applications of the Ideal Gas Law: Molar Mass, Density, and Volume03:43

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The volume occupied by one mole of a substance is its molar volume. The ideal gas law, PV = nRT,  suggests that the volume of a given quantity of gas and the number of moles in a given volume of gas vary with changes in pressure and temperature. At standard temperature and pressure, or STP (273.15 K and 1 atm), one mole of an ideal gas (regardless of its identity) has a volume of about 22.4 L — this is referred to as the standard molar volume.
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What is an Electrochemical Gradient?01:26

What is an Electrochemical Gradient?

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Adenosine triphosphate, or ATP, is considered the primary energy source in cells. However, energy can also be stored in the electrochemical gradient of an ion across the plasma membrane, which is determined by two factors: its chemical and electrical gradients.
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
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Centrifugation01:05

Centrifugation

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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Atoms — and the protons, neutrons, and electrons that compose them — are extremely small. For example, a carbon atom weighs less than 2 × 10−23 g. When describing the properties of tiny objects such as atoms, we use appropriately small units of measure, such as the atomic mass unit (amu). The amu was originally defined based on hydrogen, the lightest element, then later in terms of oxygen. Since 1961, it has been defined with regard to the most abundant isotope of carbon, atoms of which...
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Centrifugal Force01:06

Centrifugal Force

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Pseudo forces, or fictitious forces, appear to act on an object in motion in a rotating frame of reference with respect to an inertial reference frame. These forces are not real forces but rather mathematical constructs and are introduced to simplify calculations in a non-inertial frame while using Newton's laws of motion. Common examples of pseudo forces include centrifugal, Coriolis, and Euler forces. These forces are essential in fields such as mechanics, astrophysics, and fluid...
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Density00:56

Density

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Density is an important characteristic of substances, crucial in determining whether an object sinks or floats in a fluid. Its SI unit is kg/m3, and its cgs unit is g/cm3. The density of an object helps in identifying its composition, and also reveals information about the phase of the matter and its substructure. The densities of liquids and solids are roughly comparable, consistent with the fact that their atoms are in close contact. However, gases have much lower densities than liquids and...
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使用微通道梯度离心法测量单细胞质量密度.

Richard Soller1, Per Augustsson2, Rune Barnkob3,4

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此摘要是机器生成的。

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科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 微流体学 微流体学

背景情况:

  • 精确的单细胞质量密度测量对于理解细胞异质性和功能至关重要.
  • 现有的方法往往缺乏大规模分析所需的吞吐量或精度.

研究的目的:

  • 为准确和高通量单细胞质量密度测量提供质量密度梯度离心的基于微通道的适应.
  • 引入微观质密度读数的方法,并证明该技术的有效性.

主要方法:

  • 工作流程包括微通道填充,离心和显微镜.
  • 一个一维的质量密度梯度是瞬间和可重复生成的.
  • 校准颗粒和痕迹分子被用于微观质量密度读取.

主要成果:

  • 成功测量了单个酵母细胞的质量密度.
  • 该方法在质量密度测量中获得了3.3%的中位数不确定性.
  • 证明了每小时大约16000个细胞的吞吐量.

结论:

  • 基于微通道的质密度梯度离心是一种精确,高吞吐量和负担得起的单细胞分析方法.
  • 这种技术显著提高了单细胞质量密度测量的可访问性.
  • 该方法的稳定性和简单性使其适合在生物研究中广泛采用.