对果的比较转录组分析揭示了候选基因,这些基因参与了对Botryosphaeria dothidea的软腐蚀抵抗
Danni Lv1, Yuxiao Li1, Xiaoxia Zuo1
1College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, China.
Journal of the science of food and agriculture
|February 14, 2026
概括
耐药的果品种显示出更高水平的,黄和素. 参与氨酸代谢,信号传递和激素途径的差异性表达基因有助于对Botryosphaeria dothidea产生软腐蚀抵抗.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 由Botryosphaeria dothidea引起的软腐烂是果中一个重要的收获后疾病.
- 基维果品种对软腐烂的易感性各不相同,但潜在的分子机制仍然不清楚.
研究的目的:
- 调查"金" (JY) 基维果对Botryosphaeria dothidea的耐药性的分子基础.
- 确定参与果对软腐烂的防御反应的关键基因和途径.
主要方法:
- 对抗性 (JY) 和敏感性 (JK) 的果品种进行比较分析.
- 转录组测序以识别差异表达基因 (DEGs).
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
- 使用实时定量聚合酶链反应 (RT-qPCR) 验证基因表达.
主要成果:
- 与"Jinkui"相比",Jinyan"品种对B. dothidea的耐药性更高,并且,黄和素的积累更大.
- 转录组分析发现了4011个差异表达的基因,在氨酸代谢,信号传递,转录因子和植物激素信号通路中显著丰富.
- 在氨酸代谢 (例如,CHS,CCR),信号 (例如,CALM2) 和MAPK信号 (例如,MKK4_5) 中的关键基因被确定为抵抗的关键基因.
- 基因表达验证证实JY品种中耐药性相关基因的表达水平更高.
结论:
- 这项研究阐明了关键的功能基因和防御途径,使基维果对B. dothidea诱导的软腐烂产生抵抗力.
- 这些发现提供了关于不同果品种之间控制疾病耐药性的分子区别的见解.
- 已识别的基因和途径可以作为开发抗病果品种的潜在目标.
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