血液样本延长存储对血小板功能测试结果的影响:一个体外稳定性研究
Artur Hausch1, Maria Deisenhammer1, Nenad Mitrovic2
1Department of Laboratory Medicine, Hospital Voecklabruck, Austria.
Scandinavian journal of clinical and laboratory investigation
|February 14, 2026
概括
在大多数血小板功能测试中,延长血液样本存储时间长达5小时是可以接受的,除了PFA-200原蛋白/EPI测试,该测试显示了显著的偏差.
科学领域:
- 临床化学 临床化学
- 血液学 血液学 血液学
- 实验室医学 实验室医学
背景情况:
- 血小板功能测试对于评估出血风险和指导抗血小板治疗至关重要.
- 标准协议经常限制血液样本储存时间,影响实验室工作流程和患者护理.
- 了解在延长存储条件下样本的稳定性对于可靠的诊断结果至关重要.
研究的目的:
- 为了评估延长室温储存 (长达5小时) 对各种血小板功能测试的影响.
- 为了确定血小板功能测试结果的稳定性,超出制造商推的储存时间.
- 为了确定哪些特定的血小板功能测试在样本长时间存储后是可靠的.
主要方法:
- 30名患者的血液样本在采集 (基线) 后立即进行测试,并在室温下储存3-4小时和5小时后进行测试.
- 使用PFA-200 (原/EPI,原/ADP),Multiplate (ASPI,ADP,TRAP) 和VerifyNow (阿司匹林,PRU) 分析仪进行了血小板功能评估.
- 稳定性被定义为从基线值中位相对偏差 (根据测试类型<19%或<28%).
主要成果:
- 大多数测试 (原/ADP,ASPI,ADP,TRAP,阿司匹林,PRU) 显示可接受的稳定性,存储5小时后的中位数偏差低于定义限值.
- 在PFA-200原蛋白/EPI测试显示,在5小时的储存后,中位数的显著偏差为35%.
- 在原蛋白/EPI测试中,在4小时和5小时的储存之间没有发现显著差异,这表明基线测量可能存在问题.
结论:
- 血液样本可以在室温下储存长达5小时,用于几个关键的血小板功能测试,提高了实验室的灵活性.
- 在长时间储存后,PFA-200原蛋白/EPI测试结果不可靠,可能是由于测试盒的问题.
- 需要进一步调查以澄清与PFA-200原蛋白/EPI试验观察到的差异.
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