使用伪向LC-MS/MS的肺癌风险和复发相关的新兴污染物的显相分析
Guo Mengzhe1, Du Weiwei2, Wang Xiang2
1Affiliated Clinical Hospital of Xuzhou Medical University, Xuzhou Central Hospital, Xuzhou 221000, China; Collage of Pharmacology, Xuzhou Medical University, Xuzhou 221004, China.
Journal of hazardous materials
|February 14, 2026
概括
像单甲基甲酸盐 (MMPA) 这样的环境污染物与从未吸烟者肺癌风险和复发有关. 这项研究使用曝光学来识别关键化学物质及其协同作用,揭示了干预的潜在分子标.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 癌症研究 癌症研究
背景情况:
- 肺癌是导致死亡的首要原因,在从未吸烟的人群中发病率越来越高.
- 包括新兴关注的污染物 (CECs) 在内的环境暴露与此有关,但人们对其了解甚少.
- 具体的CEC与肺癌风险/预后之间的联系需要进一步研究.
研究的目的:
- 开发和应用一个敏感的曝光学平台,以对肺癌患者和对照者的血清CEC进行分析.
- 确定与肺癌发展和复发相关的特定CEC.
- 探索CEC诱导的肺癌进展背后的分子机制.
主要方法:
- 开发了一个伪定向的LC-MS/MS曝光学平台,使用97个参考标准对350个血清CEC进行分析.
- 分析了来自570名肺癌患者和307名健康对照者的血清样本.
- 利用混合物模型进行MMPA暴露的肺癌细胞的同时暴露分析和蛋白质基因分析.
主要成果:
- 检测到228个CEC,其中单甲基甲酸盐 (MMPA), perfluorooctanesulfonic酸和西马在患者中显著增加.
- 确定了MMPA,双G和Irganox 245作为与术后复发相关的关键化学物质.
- 在患者和对照人群中证实血清MMPA水平显著更高,并在暴露于MMPA的细胞中观察到途径失调.
结论:
- 整合了exposomics和proteomics来识别与肺癌相关的环境化学物质.
- 突出了联合暴露和与疾病进展和复发相关的特定CECs的协同效应.
- 提供了对导致肺癌的环境因素和潜在治疗点的新见解.
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