相关实验视频
Updated: Feb 16, 2026
06:06
In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
6.1K
HECT连接酶 HECTD3 的结构和机制
Jessica Huber1, Diego Esposito1, Sarah Maslen2
1Molecular Structure of Cell Signalling Laboratory, The Francis Crick Institute, London, UK.
Nature communications
|February 14, 2026
概括
HECTD3是一种泛素酶,其结构和功能由冷EM揭示出来. 这项研究详细介绍了其独特的机制来调节细胞过程和各种基质修饰.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- HECT E3链酶是细胞过程的关键调节者,但它们的基质识别和聚-泛胺链合成机制尚不清楚.
- "另一个HECT"家族成员HECTD3,在炎症,亡,感染中发挥作用,在癌症中表达很高,主要是由于其结合酶活性.
研究的目的:
- 综合分析HECTD3的结合酶活性,包括泛素结合偏好,寡合体状态和基质泛素化.
- 使用冷电子显微镜 (cryo-EM) 阐明HECTD3在apo和ubiquitin载荷状态中的全长结构.
主要方法:
- 对HECTD3酶活性进行详细的生物化学分析.
- 电子显微镜 (cryo-EM) 用于全长HECTD3的结构确定.
主要成果:
- 该研究提供了全长的HECTD3结构在apo和ubiquitin载荷的形式,提供了对其域组织的见解.
- 发现了独特的N端区域折叠,以及与其他HECT连接酶共享的机械特征,并且是HECTD3.3独有的.
- HECTD3表现出特定的泛素结合偏好,并修改各种基质.
结论:
- 对HECTD3的结构和机制洞察力解释了它的功能多样性和与其他HECT连接酶相比的催化差异.
- 了解HECTD3的独特特征可以了解它在癌症和其他疾病中的作用.
相关概念视频
01:58Mechanical Protein Functions
5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.7K
01:10Antibody Structure
65.8K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.8K
03:06Reaction Mechanisms
31.2K
Chemical reactions often occur in a stepwise fashion, involving two or more distinct reactions taking place in a sequence. A balanced equation indicates the reacting species and the product species, but it reveals no details about how the reaction occurs at the molecular level. The reaction mechanism (or reaction path) provides details regarding the precise, step-by-step process by which a reaction occurs.
For instance, the decomposition of ozone appears to follow a mechanism with two steps:
For instance, the decomposition of ozone appears to follow a mechanism with two steps:
31.2K
02:22Structures of Solids
18.9K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
18.9K
02:34Structural Isomerism
21.8K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.8K
03:38Structure of Lipids
99.5K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
99.5K