抗生素敏感性作为一个关键的决定因素:B. Subtilis重塑微生态学,在堆肥过程中减轻抗生素耐药性基因
Xiaoxia Hao1, Lijia Jiang1, Mengting Chen1
1Lab of Animal Ecology and Environmental Control, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China.
Bioresource technology
|February 15, 2026
概括
在堆肥过程中使用对抗生素敏感的注射剂,通过培养稳定的微生物群体,显著减少了抗生素耐药性基因 (ARG). 然而,抗生素耐药菌株增加了ARG并阻碍了堆肥效率.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 环境工程 环境工程
背景情况:
- 抗生素耐药性基因 (ARG) 构成了严重的环境威胁.
- 有氧堆肥是一种减少ARG的潜在方法.
- 在堆肥过程中对ARG降低中注射剂性质的作用尚未完全理解.
研究的目的:
- 为了研究注射剂抗生素敏感性在有氧堆肥过程中对ARG动态的影响.
- 为了比较敏感和耐药的Bacillus subtilis菌株对堆肥效率和微生物群落结构的影响.
- 阐明ARG控制背后的生态机制.
主要方法:
- 在有氧堆肥中对敏感的 (S) 和耐药的 (R) Bacillus subtilis菌株进行系统比较.
- 监测温度,成熟度指数和ARG的丰度.
- 使用测序对微生物社区进化的分析.
- 识别ARG宿主和移动遗传元素 (MGE).
- 部分最小平方路径建模 (PLS-PM) 来确定关键影响因素.
主要成果:
- 敏感菌株显著提高了堆肥效率,延长了热友性阶段,提高了成熟度.
- 耐药菌株使总ARG丰度增加了28.40%,最终ARG负担是敏感菌株的2.74倍.
- 敏感菌株促进了稳定,模块化的微生物网络,作为对ARG传播的遗传防火墙.
- 耐药菌株导致了一个脆弱的,超连接的网络,促进了横向基因转移和ARG传播.
- 与对照 (11) 和耐药 (7) 治疗相比,敏感菌株的ARG宿主显著减少 (至2个属).
- PLS-PM表明敏感菌株减少了MGE的影响,并增强了温度在ARG减少中的作用.
结论:
- 使用对抗生素敏感的功能菌株是一种可靠的策略,可以在堆肥过程中减轻环境抗生素耐药性风险.
- 消毒剂的敏感性在确定堆肥对ARG减少的有效性方面发挥着关键作用.
- 生态机制涉及微生物网络的稳定性,利基范围和移动遗传元素的控制.
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