在体外胃消化过程中预测pH和温度对猪和人类素活性的影响的工具
Fitzpatrick C J1,2,3, Freitas D1, Comi I4
1Teagasc, Moorepark Research Centre, Fermoy, Co., Cork, P61 C996, Ireland.
Scientific reports
|February 15, 2026
概括
人类素在体外不同pH值和温度条件下表现出比猪素更好的活性. 这项研究提供了酶活性和无活化的模型,改善了体外食品消化准确性.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 酶学 是一种酶学.
背景情况:
- 在体外食品消化模型对于标准化研究至关重要,通常使用猪素来模仿人类胃酶.
- 关于pH值和温度波动如何影响消化过程中的素活性的数据有限.
研究的目的:
- 研究pH (1-7) 和温度 (4-60°C) 对人类和猪素活性的影响.
- 开发不同条件下的素活性预测模型.
- 为了确定猪素的最佳失活条件.
主要方法:
- 在pH值范围为1-7,温度为4-60°C的范围内评估素活性.
- 开发酶活动的预测数学模型.
- 测试猪素的热失活协议.
主要成果:
- 在经过测试的pH和温度范围内,人类素的活性比猪素更高.
- 为人类和猪素活性建立了预测模型.
- 猪素需要75°C5分钟以上才能完全失活,较低的温度是不够的.
结论:
- 人类素在体外表现出比猪素更高的稳定性.
- 开发的模型和无活化数据提高了体外消化研究的可靠性和可比性.
- 这些发现支持食品研究的准确性提高,并将体外结果转化为体外人类消化.
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