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清除丸的无标签高分辨率体积成像

Marwa Moulzir¹, Doriane Hazart¹, Brigitte Delhomme¹

¹Université Paris Cité, SPPIN, Saints-Pères Paris Institute for the Neurosciences, CNRS, Paris, France.

Reproduction (Cambridge, England)

|February 16, 2026

概括

我们开发了一种快速,无标签的3D成像方法,用于小鼠丸,使用自光和光学清除. 这种技术准确地可视化丸结构和细胞类型,推进生殖研究.

科学领域:

生殖生物学生殖生物学
生物医学成像技术生物医学成像技术
历史学历史学历史学

背景情况:

精确的丸组织表征对于诊断生殖障碍和恶性瘤至关重要.
传统的组织学方法通常涉及切割和染色,这可以引入文物并限制3D可视化.

研究的目的:

提出一种快速,无标签的3D成像技术,用于全面的小鼠丸表征.
为了可视化厚丸样本的完整3D结构,而无需切割.

主要方法:

使用内源性自光作为天然对比剂.
采用快速,无毒的光学清除协议,用于厚组织样本.
利用高分辨率显微镜技术,包括图像扫描共聚焦显微镜 (AiryScan).

主要成果:

成功揭示了小鼠丸样本的完整3D架构.
清晰地区分了上皮管,间歇组织和关键细胞类型 (细菌,塞尔托利,莱迪格) 的高精度.
证明了自光作为多重分析的有效结构反污染剂.

结论:

开发的方法提供精确的,高分辨率的丸组织3D成像.
这种无标签的方法避免了传统的组织学文物,并且与标准的共聚焦显微镜兼容.
该技术使先进的丸成像实现了民主化,加速了生殖研究的进展.

关键词:

自动光是一种自发光.与焦点一致的检测检测组织学组织学组织学图像扫描显微镜扫描显微镜没有标签的无标签.形态分析分析形态分析频谱分析是一种分析.整体器官清算整体器官清算

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