相关实验视频

Updated: Feb 17, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

精子DNA碎片化 (SDF) 在预测生殖结果中的价值 - 系统性审查和元分析

Srisailesh Vitthala¹, Silvia Nedelcu², Tarek El-Toukhy³

¹University of Aberdeen, Aberdeen, UK.

Human fertility (Cambridge, England)

|February 16, 2026

概括

精子DNA碎片化 (SDF) 测试显示,男性预测怀孕或流产的能力有限. 高的SDF水平具有低灵敏度和高特异性,仅仅表明预测价值较差.

科学领域:

生殖医学生殖医学
安德罗学与人类学
遗传学是一个遗传学.

背景情况:

精液分析是男性不孕症诊断的标准,但往往缺乏准确性.
精子DNA碎片化 (SDF) 测试正在成为一种补充诊断工具.
高水平的SDF与不良的生殖结果有关.

研究的目的:

系统地审查和元分析高SDF的诊断准确度,以预测怀孕和流产.
评估SDF在自然受孕和辅助生殖技术 (ART) 中的预测性能.

主要方法:

在2025年3月之前发表的52项试验 (19,930名受试者) 的系统综述和元分析.
在多个数据库中搜索使用COMET,SCSA,TUNEL或SCD测定报告怀孕或流产的研究.
通过测试截止值提取到2x2表中的数据;用随机效应HSROC模型进行分析.

主要成果:

高SDF表现出低灵敏度和高特异性,用于预测自然和辅助受孕之间的怀孕和流产.
概率和诊断几率比率表明歧视能力有限.
观察到显著的异质性,HSROC曲线显示差到中等的歧视.

结论:

高SDF测试本身对于预测怀孕或流产的临床价值有限.
需要进一步进行精心设计的试验,以确定SDF测试的临床实用性.
在男性不孕症诊断中SDF的作用需要进一步调查.

关键词:

精子DNA碎片化测定/测试 (COMET/TUNEL/SCSA/SCD) 是一种方法.流产流产流产流产怀孕 (活产/正在进行的怀孕)精子DNA损伤精子DNA损伤

更多相关视频

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

相关概念视频

Infertility in Males

Infertility in Males

Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...

Sperm Structure and Semen Composition

Sperm Structure and Semen Composition

During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...