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Updated: Feb 17, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

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一结合PCR用于检测通用RNA生物标志物的PCR.

Jinding Liu1, Zhixin Xie1, Nini Li1

  • 1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, Hebei, P. R. China. yqcheng@hbu.edu.cn.

The Analyst
|February 16, 2026
PubMed
概括

这项研究引入了一种用于检测RNA生物标志物的新型单结合-PCR试验. 与传统技术相比,简化方法提高了准确性并降低了污染风险.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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