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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

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ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.8K
Genomics02:02

Genomics

40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

24.7K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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相关实验视频

Updated: Feb 17, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

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InspectorORF:用于可视化Ribo-Seq和其他基因组或转录基因组数据的工具.

Eilidh L Ward1,2, Isabel Birds1,2, Mary J O'Connell3,4

  • 1School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.

Bioinformatics advances
|February 16, 2026
PubMed
概括
此摘要是机器生成的。

InspectorORF是一个新的R包,可视化核糖体分析和RNA测序数据. 这个工具有助于验证新的开放阅读框架 (ORF) 和理解翻译.

更多相关视频

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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相关实验视频

Last Updated: Feb 17, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 核糖体概况分析对翻译体的理解有了进步,揭示了许多替代的开放阅读框架 (ORF).
  • 目前的ORF检测工具缺乏视觉检查功能,这对于验证和预测翻译至关重要.
  • 新型ORF包括外,重叠,上游,下游和非正规的起始密码子变体.

研究的目的:

  • 为了呈现InspectorORF,一个用于可视化核糖体分析和RNA测序的R包读取了.
  • 通过对翻译数据进行视觉检查,促进新型ORF的验证和预测.
  • 整合多样化的证据,包括蛋白质组学和长读序列,以进行强大的ORF验证.

主要方法:

  • 开发InspectorORF,一个用于数据可视化的R包.
  • 结合核糖体分析和RNA测序,可以进行比较分析.
  • 可定制的绘图选项,包括蛋白质和长读序列数据.

主要成果:

  • 检查员ORF使得转录和翻译信号之间的区别.
  • 该包通过集成的数据可视化来促进预测的新型ORF的验证.
  • 来自蛋白质组学和长读序列的补充证据可以用于增强验证.

结论:

  • InspectorORF为研究人员研究翻译和新 ORF 发现提供了宝贵的工具.
  • 对核糖体分析数据的视觉检查对于准确的ORF验证至关重要.
  • 多omics数据的综合分析提高了新型ORF识别的可靠性.