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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

25.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.8K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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相关实验视频

Updated: Feb 18, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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人类信使RNA的港口广泛的非编码拼接异型.

Qili Shi1, Haochen Li1, Junjiao Song1

  • 1Fudan University Shanghai Cancer Center and Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, 270 Dong An Road, Xuhui District, Shanghai 200032, China.

Briefings in bioinformatics
|February 16, 2026
PubMed
概括
此摘要是机器生成的。

人类mRNA含有众多非编码拼接异型,通过替代拼接和多基化生成. 这些调节性异型在癌症中很普遍,可以预测患者的生存率,揭示mRNA.

关键词:
它们是mRNARNA.mRNA翻译非编码二元性没有编码的拼接异形.蛋白质编码的异型蛋白.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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相关实验视频

Last Updated: Feb 18, 2026

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 来自蛋白质编码基因的前传者RNA通常经历替代拼接,产生各种蛋白质异型.
  • 从蛋白质编码基因中衍生出来的人类mRNA中存在非编码拼接异型的存在以前是未经探索的.

研究的目的:

  • 调查人类mRNA中非编码拼接异型的流行率和特征.
  • 探索这些非编码异型体在组织特异性,RNA处理和癌症中的作用.

主要方法:

  • 人类转录基因数据的生物信息分析.
  • 识别和表征mRNA非编码异型.
  • 组织分布的分析,与RNA处理途径的关联,以及癌症相关性.

主要成果:

  • 在7298个人类蛋白质编码基因中发现了15836种mRNA非编码异型.
  • 这些异构体主要通过替代拼接和多化产生,表现出组织特异性表达.
  • 癌症中mRNA非编码异型的升级,与癌症特征的关联,以及患者生存的预测价值.

结论:

  • 人类mRNA含有广泛的非编码拼接异型,除了蛋白质编码的外.
  • 这些非编码异型体起着调控作用,并与癌症进展有关.
  • 突出了mRNA的功能双重性,既适应蛋白质翻译和调控非编码功能.