相关实验视频

Updated: Feb 18, 2026

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

Nrf2 调节了在血管损伤后新阴位形成的初始阶段的单细胞/巨细胞的招募

Takashi Ashino¹, Masayuki Yamamoto², Satoshi Numazawa¹

¹Department of Toxicology, Showa Medical University Graduate School of Pharmacy, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.

Free radical biology & medicine

|February 16, 2026

概括

在血管损伤后,Nrf2系统调节巨细胞的招募. Nrf2 缺乏会增强单细胞/巨细胞的粘附和化学反应,导致动脉样硬化发展.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

单细胞/巨细胞的招募在血管损伤后启动动动脉硬化.
Nrf2系统调节氧化应激反应和血管重塑.
Nrf2在受伤后早期单细胞/巨细胞招募中的作用尚不清楚.

研究的目的:

研究Nrf2系统在血管损伤后的单细胞/巨细胞招募和粘附中的作用.
确定Nrf2在新极度扩张的初始阶段的功能意义.

主要方法:

使用了机械动脉损伤的小鼠模型.
在内脏区域内的巨细胞 (F4/80+) 中评估Nrf2表达.
研究了Nrf2缺乏对单细胞化学吸引蛋白-1 (MCP-1) 刺激的巨细胞行为的影响.
通过使用野生型 (WT) 和Nrf2-缺乏 (Nrf2-/-) 的小鼠进行了骨髓移植研究.

主要成果:

在受伤后7天内,Nrf2在内巨细胞中表达高.
MCP-1促进了Nrf2核转位,并诱导了基因.
Nrf2缺乏症加剧了MCP-1-刺激的巨细胞化学反应和反应性氧物种的产生.
在受伤后的Nrf2-/-小鼠中,巨细胞的招募和粘附增强.

结论:

Nrf2系统在MCP-1刺激的单细胞/巨细胞的招募和粘附中起着调节作用.
Nrf2 影响了经过新极度损伤后的早期血管改造过程.
准Nrf2通路可能为动脉硬化提供治疗策略.

关键词:

Nrf2 没有任何问题.单细胞化疗吸引蛋白-1-1 一个单细胞/巨细胞.氧化应激是一种氧化应激.血管损伤是因为血管损伤.血管静止症是指血管静止症.

更多相关视频

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Published on: May 7, 2021

相关概念视频

Inflammation

Inflammation

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...