可代谢蛋白质供应对中期哺乳期奶牛中CD4+T淋巴细胞代谢功能的影响
U Arshad1, T Fernandez-Wallace1, R Fukumori1
1Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison 53706.
Journal of dairy science
|February 16, 2026
概括
乳牛的低最佳氨酸和蛋白质 (MP) 供应降低了CD4+ T淋巴细胞代谢功能和持久性. 这种代谢障碍可能会在早期哺乳期等营养不平衡期间损害免疫反应.
科学领域:
- 动物科学动物科学
- 免疫学 免疫学 免疫学
- 营养生物化学 营养生物化学
背景情况:
- 中期哺乳期的奶牛面临的营养挑战可能会影响免疫细胞功能.
- CD4+ T 淋巴细胞对于适应性免疫至关重要,其代谢状态影响其有效性.
- 食蛋白质供应是影响牛整体健康和免疫能力的关键因素.
研究的目的:
- 研究不同氨酸和蛋白质 (MP) 供应对乳牛中非活化 (NA) 和活化 (AC) CD4+ T 淋巴细胞代谢功能的影响.
- 确定MP供应不足是否会影响CD4+T细胞中的线粒体呼吸和糖解.
- 评估MP供应对T细胞持久性和ATP生产的影响.
主要方法:
- 32只中期哺乳期的荷尔斯坦牛在12周内接受了MP充足或不足的饮食.
- 分离了周围血液单核细胞,随后磁性分离了CD4+ T淋巴细胞.
- 通过在NA和AC条件下的细胞外流量分析,评估了代谢功能 (氧气消耗率,细胞外酸化率,质子外流率).
主要成果:
- 养不充分的MP饮食的奶牛在NA和ACCD4+T淋巴细胞中表现出较低的氧气消耗率和最大呼吸率.
- 不足够的MP供应导致有氧糖解和T细胞持续性随着时间的推移逐渐下降.
- 虽然总ATP产生没有受到影响,但与NA CD4+ T淋巴细胞相比,在AC中,糖性ATP的产生更高.
结论:
- 低于最佳的MP供应损害了乳牛中CD4+T淋巴细胞的代谢适应性和持久性.
- 由于营养不平衡,T细胞的代谢功能下降可能会影响免疫反应.
- 维持足够的MP供应对于在营养压力时期支持免疫细胞功能至关重要.
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