杂交内体外层含有不同类别的分类nexins
Navin Gopaldass1, Sudeshna Roy Chowdhury2, Ana Catarina Alves2
1Department of Immunobiology, University of Lausanne, Epalinges, Switzerland. navin.gopaldass@unil.ch.
The EMBO journal
|February 16, 2026
概括
排序nexins (SNXs) 在内分泌体上形成混合层,使可适应性蛋白质运输成为可能. 这些新的SNX-BAR和Snx3复合体优化了对各种蜂目的地的货物分类.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 贩卖蛋白质 贩卖蛋白质 是一个问题.
背景情况:
- 内基因组作为关键的蛋白质分类中心.
- 不同的分类nexin (SNX) 类为特定的内分体分类路径形成同质的层.
- SNX-BAR蛋白和Snx3代表不同的SNX类,具有不同的功能.
研究的目的:
- 研究由SNX-BAR蛋白和Snx3.3蛋白组成的混合层的形成和功能.
- 为了确定混合涂层与同质涂层相比是否表现出不同的特性.
- 了解共同表达的SNX-BARs和Snx3.3的体内影响.
主要方法:
- 净化SNX-BARs,Snx3,以及货物蛋白质.
- 在试验室组装均和混合外套.
- 对外层结构,膜协会和脚手架活动的分析.
- 在生物共定位研究和货物分类分析.
主要成果:
- 由SNX-BARs和Snx3形成的混合层组装具有可变的子单位比和直径.
- 混合涂层与均质涂层相比,显示了增强的膜支架活动.
- 在体内,Snx3和SNX-BAR共同定位并影响对方的货物分类.
- 混合外套将两种SNX类型纳入一个共同的复合体,可能是通过逆转分子寡合化.
结论:
- SNX-BARs和Snx3形成具有可适应的固态度的逆相介导混合层.
- 这些混合外套提供了一个调整内体体载体以适应不同载荷比率的机制.
- 这一发现揭示了内体蛋白分类中的新型调节层.
相关概念视频
Clathrin Coated Vesicles
9.5K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.5K
COP Coated Vesicles
18.3K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
18.3K
Coat Assembly and GTPases
4.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.5K
Maturation of Endosomes
6.0K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
6.0K
Nuclear Protein Sorting
6.5K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.5K
The Early Endosome: Endocytosis of Transferrin
4.9K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.9K


