相关实验视频

Updated: Feb 18, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

用多酶消化策略与LC-HRMS工作流程集成的片映射:一个案例研究

Deep Maheshwari¹, Devendra Badgujar¹, Gulshan Kumar¹

¹Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), India.

Journal of peptide science : an official publication of the European Peptide Society

|February 17, 2026

概括

一个新的多酶映射工作流程增强了疗法的结构验证. 这种方法实现了关键抗糖尿病的完整序列覆盖,确保了准确的初级结构评估.

科学领域:

药品分析药品分析
生物化学生物化学
分析化学分析化学

背景情况:

类治疗药物具有显著的治疗潜力,但由于结构复杂性而存在发展挑战.
先进的分析技术,包括基映射,对于识别基主要结构和证明结构相似性至关重要.
像FDA和EMA这样的监管机构建议片映射用于结构验证.

研究的目的:

描述一种新型的多酶映射工作流程,旨在改善疗法的序列覆盖和结构验证.
用exenatide和H-GLP-1作为模型系统来证明工作流的有效性.

主要方法:

整合多酶消化与互补的特异性,以生成多样化的图.
使用Waters XBridgeBEH C18柱和梯度化进行分离.
高分辨率液体色谱-质谱 (LC-HRMS) 与轨道探测器用于精确的片段识别 (<5 ppm质量精度).

主要成果:

通过结合用素,Glu-C和化学素生成的基图来实现exenatide和H-GLP-1的完整序列覆盖.
应用于GLP-1模拟的工作流产生了超过95%的序列覆盖率,并确认了完整的质量.
使用高分辨率的MS/MS数据获得了精确的片段识别.

结论:

描述的多酶映射工作流程为疗法的结构验证提供了一种可靠和逐步的方法.
这种方法可以对像exenatide和H-GLP-1这样的的初级结构进行可靠的评估.
工作流程增强了序列覆盖和结构确认,解决了类药物开发中的关键挑战.

关键词:

在GLP-1的模拟中.在 LC-Orbitrap 中.在 RP-UPLC-HRMS 中.生物信息工具是生物信息工具.类映射绘制的类映射

更多相关视频

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...