代谢重编程和GM-CSF分泌在阿雷卡坚果激活纤维细胞驱动口腔癌前期进展
Yen-Yun Wang1,2,3, Chang-Wei Su1,4, Chih-Huang Tseng1,5
1School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Oral diseases
|February 17, 2026
概括
阿拉卡坚果提取物 (ANE) 通过激活纤维细胞,增加表皮细胞到介质细胞的过渡,并增强癌前细胞中的表皮生长因子受体 (EGFR) 信号传递,促进口腔癌.
科学领域:
- 口腔病理学 口腔病理学
- 癌症研究 癌症研究
- 纤维细胞生物学
背景情况:
- 全球口腔癌的发病率正在上升,特别是在南亚/东南亚,与果使用有关.
- 阿拉卡坚果提取物 (ANE) 是一种可疑的致癌物,但其在口腔组织上的机制需要阐明.
- 纤维细胞行为调节是口腔致癌的一个潜在因素.
研究的目的:
- 为了研究阿雷卡坚果提取物 (ANE) 对口腔致癌的作用.
- 确定ANE如何调节口腔组织中的纤维细胞行为.
- 探索纤维细胞激活在口腔癌前病变进展中的作用.
主要方法:
- 通过基于细胞的测试评估纤维细胞活力,迁移和入侵.
- 利用细胞因子阵列,ELISA,基酶阵列和西式涂抹来分析细胞功能.
- 在人类和仓鼠口腔组织中使用免疫组织化学和免疫光学进行验证.
主要成果:
- ANE增加了纤维细胞线粒体新陈代谢和诱导肌纤维细胞过渡,由增加的α-SMA和纤维细胞激活蛋白 (FAP) 证明.
- ANE增强了表皮细胞到介质细胞的过渡和纤维细胞中颗粒细胞-巨细胞殖民地刺激因子 (GM-CSF) 的分泌.
- 经ANE处理的纤维细胞和GM-CSF促进了表皮生长因子受体 (EGFR) 酸化和恶性转化在失塑性口腔角质细胞中.
结论:
- 阿拉卡坚果提取物 (ANE) 通过促进纤维细胞激活和上皮转移到介质酶的过程来驱动口腔致癌.
- 纤维细胞的ANE诱导的GM-CSF分泌激活了邻近的上皮细胞中的EGFR信号.
- 这种由ANE介导的纤维细胞-上皮细胞交叉作用有助于口腔癌前细胞的恶性转变.
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