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Updated: Feb 19, 2026

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结构适应的双重功能聚-T 序列使一个均的无标签的色度测量传感平台成为可能

Shao-Yu Sun¹, Yi Song¹, An-Qi Xiao²

¹School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China. zhouqy@cqust.edu.cn.

|February 17, 2026

概括

聚胺 (poly-T) DNA 序列显示出类似过氧化酶的活性,使新的色度传感器成为可能. 这个传感器通过观察DNA的变化来检测胺.

科学领域:

生物化学生物化学
分子生物学分子生物学
纳米技术纳米技术

背景情况:

对富含G的核酸的研究广泛,但富含T的序列的催化性质仍未得到充分探索.
聚胺 (poly-T) 序列通常被视为它们的结构作用,而不是催化功能.

研究的目的:

为了研究聚-T序列的过氧化酶类活性.
开发一种新的同质色度传感平台,用于使用聚-T序列检测胺.

主要方法:

研究了用乙酸作为激活剂的多-T序列的过氧化酶类活性.
构建了一个均的色度测量传感平台,利用长链聚-T序列来催化TMB-H2O2反应.
利用T-胺-T结构的形成来诱导形状变化并抑制催化活性.

主要成果:

聚-T 序列表现出类似过氧化酶的活性,效率随着序列长度的增加而增加.
开发的传感平台成功检测了胺.
胺与多T序列的结合导致结构变化,显著抑制了催化活性.

结论:

聚-T 序列具有固有的催化特性,作为可激活的过氧化酶模仿剂.
一个新的,双功能的ssDNA传感平台集成了催化和识别功能,用于检测胺.
聚-T序列的结构依赖性活性调制允许通过色度测试快速地在现场检测.

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