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内在的细胞异质性指导不同的表皮细胞到介质酶细胞过渡结果
Esin Ozkuru Sekeroglu1, Şeyma N Yıldız1, Seray Yetkin1
1Izmir International Biomedicine and Genome Institute, Dokuz Eylül University, Turkey.
The FEBS journal
|February 17, 2026
概括
细胞异质性显著影响表皮细胞到介质酶细胞过渡 (EMT) 的结果. 在癌症研究中,了解内在的上皮质身份对于预测疾病进展和转移性行为至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 癌症研究 癌症研究
背景情况:
- 表皮细胞到介质细胞过渡 (EMT) 对于发育和疾病至关重要,但其研究受到表皮细胞模型异质性的阻碍.
- 预先存在的细胞变异性会影响EMT期间对转化生长因子β3 (TGFβ3) 的不同反应.
研究的目的:
- 调查内在表皮质异质性如何影响EMT动态和结果.
- 探索细胞身份在塑造EMT轨迹和转移潜力的作用.
主要方法:
- 流细胞计,基因表达分析和单细胞成像用于分析上皮细胞异质性.
- 采用了亚种群和单细胞衍生克隆的差分分类.
- 在体内使用斑马鱼异种移植模型来评估传播潜力.
主要成果:
- 观察到E-cadherin (E-cad) 和EpCAM表达的持续异质性,在排序的亚群中具有不同的转录反应.
- 一些克隆在EMT期间显示出矛盾的CDH1上调,与GRHL3诱导相关,表明特定环境的可塑性.
- 福斯科林介导的cAMP激活增强了上皮的稳定性和调节了EMT动态.
- 内在的上皮特征与体内差异性传播潜力相关.
结论:
- 固有的细胞异质性极大地影响了EMT轨迹和疾病进展.
- 考虑到内在的上皮质身份及其与外在信号的相互作用对于准确的EMT建模至关重要.
- 细胞身份影响转移性行为,突出其在癌症研究中的重要性.
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